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:: Volume 8, Issue 8 (1-2012) ::
3 2012, 8(8): 0-0 Back to browse issues page
Pen-Yuan Chu * , Fang-Wei Hu , Cheng-Chia Yu , Lo-Lin Tsai , Chuan-Hang Yu , Buor-Chang Wu , Yi-Wei Chen , Pin-I Huang , Wen-Liang Lo
Department of Otolaryngology, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan,Division of Urology, Department of Surgery, Taipei Veterans General Hospital—Su-Ao/Yuan-Shan Branch, Taipei, Taiwan,Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan
Abstract:   (1839 Views)

 Objectives

 Methods Tumor initiating capability of HNC-CH133+ cells with ZEB1/2 knockdown or co-overexpression was presented in vitro and in vivo.

 Results In the present study, we demonstrated that ZEB1/ZEB2 expression was significantly increased in HNC-CD133+ CSC-like cells compared with HNC-CD133− cells. The small interfering RNA (siRNA)-mediated co-knockdown of ZEB1 and ZEB2 (siZEB1/2) in HNC-CH133+ cells suppressed their CSC-like properties, including self-renewal ability, the expression of stemness markers, and drug resistance. In contrast, the co-overexpression of ZEB1/ZEB2 in HNC-CD133− cells enhanced their sphere-forming ability and increased the percentage of CD44-positive cells and side population cells. In vivo studies showed that the delivery of siZEB1/2 to xenograft tumors in nude mice reduced tumor growth and the rate of distant metastasis. In clinical samples, the levels of ZEB1/ZEB2 expression were low in local lesions but high in metastatic lymph nodes in HNC tissues. Patients with tumors that co-expressed ZEB1high and ZEB2high had especially poor survival rates.

 Conclusion Therapies targeting ZEB1/ZEB2 in HNC-CD133+ cells may provide a new approach for HNC therapy in the future.


 Source: Journal of Oral Oncology

 Full Text

Both epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties may be involved in metastasis, which contributes to the high mortality rate of patients with head and neck cancers (HNCs). However, the mechanisms through which the EMT transcription factors ZEB1 and ZEB2 regulate HNC are still unclear.
Keywords: ZEB1, ZEB2, EMT, Cancer stem cells, Head and neck cancer
     
Type of Study: Research | Subject: Oral and Maxillofacial Surgery
Received: 2012/12/24 | Published: 2012/01/15
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Volume 8, Issue 8 (1-2012) Back to browse issues page
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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