Objectives MicroRNAs (miRNAs) are endogenous small non-coding RNAs that negatively regular target gene expression by RNA interference. The processing of the pre-miRNA hairpin generates a miRNA duplex, which consists of a miRNA (guide strand) and a miRNA (passenger strand). miR-31 is an oncogenic miRNA and is up-regulated in oral squamous cell carcinoma (OSCC). miR-31 shows a high level of conservation across species and, based on this, this study hypothesized that miR-31 is a functional miRNA.

 Materials and Methods The expression of miR-31 and miR-31 in OSCC tissues and oral cells were analyzed. Functional studies were performed on OSCC cells.

 Results miR-31 is up-regulated in OSCC tissues, but its expression is less abundant than miR-31. miR-31 decreases the proliferation and migration of both SAS and Fadu cells. Furthermore, miR-31 targets the 3′UTR of RhoA and is able to down-regulate RhoA expression. Knockdown of RhoA expression is known to decrease the proliferation and migration of OSCC cells. However, up-regulation of both miR-31 and miR-31 by delivery of pre-mir-31 does still enhance OSCC oncogenicity.

 Conclusion miR-31 is a functional miRNA involving in regulating RhoA, and the activity of miR-31’s activity seems to counteract the functions of miR-31 during OSCC tumorigenesis.

 Source: Journal of Oral Oncology

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