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:: Volume 2, Issue 2 (1-2012) ::
3 2012, 2(2): 0-0 Back to browse issues page
Comparing the Effects of Mineral Trioxide Aggregate and Calcium Enriched Mixture on Neuronal Cells Using an Electrophysiological Approach
Fatemeh Abbasipour , Vahid Akheshteh , Ali Rasteqar , Habib Khalilkhani , Saeed Asgari , Mahyar Janahmadi *
Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (3193 Views)

  Introduction: The main goal of this ex vivo study was to assess and compare the cellular and electrophysiological effects of two dental biomaterials, white mineral trioxide aggregate (WMTA) and calcium enriched mixture (CEM) cement, on neuronal cell excitability and electrical properties.

  Materials and Methods: A conventional intracellular current clamp technique was used to study the cellular effects of WMTA and CEM on the excitability, firing and the shape of action potential of neuronal soma membrane of F1 nerve cells. The dental biomaterials were prepared according to the manufacturers' directions and were applied to the bathing media and 0.05 mL of total mixture of each dental material at a distance of 3 mm from the cells.

  Results: Findings indicated that exposure to both dental biomaterials shifted the irregular high frequency firing type observed in control conditions to a more regular low frequency firing pattern. Neuronal exposure to WMTA, but not CEM, significantly hyperpolarized the cell resting membrane potential. Both treatments significantly influenced the duration and the amplitude of action potentials. Extracellular application of either CEM or WMTA caused a significant increase in the after hyperpolarization (AHP) amplitude and AHP area, but the potentiating effect of WMTA was more effective than CEM.

 Conclusion: Treatment with WMTA or CEM resulted in a profound alteration in the firing behaviour of F1 cells and changed the AP characteristics. Both dental biomaterials reduced the neuronal activity possibly through enhancement of K+ outward current. This may possibly explain the positive mechanisms of these biomaterials in regenerative endodontics, though further research is needed for such a conclusion.


  Source: Iranian Endodontic Journal

  Full text

Keywords: Action Potential; Calcium Enriched Mixture; CEM cement; Helix Aspersa; Intracellular Recording; MTA; Neuronal; White Mineral Trioxide Aggregate
     
Type of Study: Research | Subject: Endodontics
Received: 2012/05/6 | Published: 2012/01/15
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Abbasipour F, Akheshteh V, Rasteqar A, Khalilkhani H, Asgari S, Janahmadi M. Comparing the Effects of Mineral Trioxide Aggregate and Calcium Enriched Mixture on Neuronal Cells Using an Electrophysiological Approach. 3 2012; 2 (2)
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Volume 2, Issue 2 (1-2012) Back to browse issues page
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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