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:: دوره 6، شماره 6 - ( پریودنتولوژی 1390 ) ::
جلد 6 شماره 6 صفحات 0-0 برگشت به فهرست نسخه ها
A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression
چکیده:   (3938 مشاهده)

 Background: In the present study, the expression and localization of three epithelial peptides (human β-defensin [hBD]-2 and -3, and cathelicidin [LL-37]) are studied in an organotypic dento-epithelial (OD-E) model exposed to Fusobacterium nucleatum (Fn) biofilm.

 Methods: Biofilm of Fn ATCC 25586 or AHN 9508 were produced by culturing each strain on semipermeable membranes. The OD-E model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pieces on the top. A 3-day-old biofilm was placed on the top of the OD-E and the coculture was incubated for 5 hours or 24 hours. Production of epithelial antimicrobial peptides was determined immunohistochemically.

 Results: After 5 hours of incubation, the biofilm of each Fn strain stimulated expression of hBD-2 and -3. hBD-2 was localized on superficial layers and hBD-3 on basal cell layers of the epithelium and dento-epithelial junctions, whereas LL-37 was only weakly expressed. After 24 hours, hBD-2 expression was extended toward basal cell layers of the epithelium. In contrast, hBD-3 expression extended toward superficial layers of the epithelium. In the case of Fn AHN 9508 biofilm, LL-37 was localized in the cell layers of the dento-epithelial junction.

 Conclusion: In our OD-E model, epithelial antimicrobial peptide responses to Fn biofilms have distinct regulation and localization characteristics, resembling those known to occur in the gingival epithelium in vivo.


  Source : Journal of Periodentology

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نوع مطالعه: پژوهشي | موضوع مقاله: پریودنتولوژی
دریافت: 1390/11/24 | انتشار: 1390/10/25
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K. Gursoy U, Pöllänen M, Könönen E, Uitto V. A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression. 3 2012; 6 (6)
URL: http://idai.ir/article-1-295-fa.html

A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression. 1. 1390; 6 (6)

URL: http://idai.ir/article-1-295-fa.html



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Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
دوره 6، شماره 6 - ( پریودنتولوژی 1390 ) برگشت به فهرست نسخه ها
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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