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Time-kill behaviour against eight bacterial species and cytotoxicity of antibacterial monomers
چکیده:   (1975 مشاهده)

 Objectives

  The objectives of this study were to investigate: (1) the antibacterial activity of two antibacterial monomers, dimethylaminododecyl methacrylate (DMADDM) and dimethylammoniumethyl dimethacrylate (DMAEDM), against eight different species of oral pathogens for the first time (2) the cytotoxicity of DMAEDM and DMADDM.

 Methods

  DMAEDM and DMADDM were synthesized by reacting a tertiary amine group with an organo-halide. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against eight species of bacteria were tested. Time-kill determinations were performed to examine the bactericidal kinetics. Cytotoxicity of monomers on human gingival fibroblasts (HGF) was assessed using a methyl thiazolyltetrazolium assay and live/dead viability assay.

 Results

  DMADDM showed strong bactericidal activity against all bacteria, with MIC of 1.2–9.8μg/mL. DMAEDM had MIC of 20–80mg/mL. Time-kill determinations indicated that DMADDM and DMAEDM had rapid killing effects against eight species of bacteria, and eliminated all bacteria in 30min at the concentration of 4-fold MBC. Median lethal concentration for DMADDM and DMAEDM was between 20 and 40μg/mL, which was 20-fold higher than 1–2μg/mL for BisGMA control.

 Conclusions

  DMAEDM and DMADDM were tested in time-kill assay against eight species of oral bacteria for the first time. Both were effective in bacteria-inhibition, but DMADDM had a higher potency than DMAEDM. Different killing efficacy was found against different bacteria species. DMAEDM and DMADDM had much lower cytotoxicity than BisGMA. Therefore, DMADDM and DMAEDM are promising for use in bonding agents and other restorative/preventive materials to combat a variety of oral pathogens.


 Source: Journal of Dentistry

 Full Text

واژه‌های کلیدی: Antibacterial activity، Cytotoxicity، Oral bacteria، Quaternary ammonium methacrylate، Time-kill، Human fibroblasts
     
نوع مطالعه: پژوهشي | موضوع مقاله: ترمیمی
دریافت: 1392/7/21 | پذیرش: 1394/9/25 | انتشار: 1394/9/25
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Time-kill behaviour against eight bacterial species and cytotoxicity of antibacterial monomers. 1. 1390;

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Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
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جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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