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:: Volume 6, Issue 6 (1-2012) ::
3 2012, 6(6): 0-0 Back to browse issues page
Kaempferol Inhibits P. intermedia Lipopolysaccharide-Induced Production of Nitric Oxide Through Translational Regulation in Murine Macrophages: Critical Role of Heme Oxygenase-1-Mediated ROS Reduction
In Soon Choi , Eun-Young Choi , Ji-Young Jin , Hae Ryoun Park , Jeom-Il Choi , Sung-Jo Kim *
Department of Periodontology, School of Dentistry, Pusan National University/Biomedical Research Institute, Pusan National University Hospital, Busan, Korea
Abstract:   (1757 Views)

 Background: Nitric oxide (NO) could be a potential target for the development of new therapeutic approaches to the treatment of periodontal disease because this molecule plays a significant role in the tissue destruction observed in periodontitis. In this study, the authors investigate the effect of kaempferol on the production of NO by murine macrophage-like RAW264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease, and try to determine the underlying mechanisms of action.

 Methods: NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Real-time polymerase chain reaction was performed to quantify inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) mRNA expression. iNOS and HO-1 protein expression and phosphorylation of c-Jun N-terminal kinase and p38 were characterized via immunoblot analysis. Reactive oxygen species (ROS) production was measured using the redox-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate.

 Results: Kaempferol significantly inhibited NO production and expression of iNOS protein in P. intermedia LPS-stimulated RAW246.7 cells without affecting iNOS mRNA expression. Kaempferol upregulated HO-1 expression in LPS-activated cells. Inhibition of HO-1 activity by tin protoporphyrin IX (SnPP) abolished the suppressive effect of kaempferol on NO production. In addition, kaempferol significantly attenuated P. intermedia LPS-induced increase of intracellular ROS, and SnPP blocked this reduction. Treatment with antioxidants downregulated the production of LPS-induced NO.

  Conclusions: Kaempferol inhibits NO production and iNOS protein expression in P. intermedia LPS-stimulated RAW264.7 cells at the translational level via HO-1-mediated ROS reduction and could be an efficient modulator of host response in the treatment of periodontal disease.


 Source: Journal of Periodentology

 Full Text

Keywords: Heme oxygenase-1, kaempferol, lipopolysaccharides, nitric oxide, Prevotella intermedia, reactive oxygen species
     
Type of Study: Research | Subject: Periodontology
Received: 2013/07/13 | Published: 2012/01/15
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Choi I S, Choi E, Jin J, Park H R, Choi J, Kim S. Kaempferol Inhibits P. intermedia Lipopolysaccharide-Induced Production of Nitric Oxide Through Translational Regulation in Murine Macrophages: Critical Role of Heme Oxygenase-1-Mediated ROS Reduction. 3 2012; 6 (6)
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Volume 6, Issue 6 (1-2012) Back to browse issues page
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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