[صفحه اصلی ]   [Archive] [ English ]  
جستجو در پایگاه

جستجوی پیشرفته
..
نظرسنجی
مطالب کدام بخش سایت بیشتر مورد نیاز شماست؟
پرسش و پاسخ
جشنواره شهید هدایت
کلینیک تخصصی دندانپزشکی
مجله علمی
نمایشگاه کتاب
نمایشگاه مواد و تجهیزات دندانپزشکی
نیازمندیها
   
..
:: دوره 6، شماره 6 - ( پریودنتولوژی 1390 ) ::
جلد 6 شماره 6 صفحات 0-0 برگشت به فهرست نسخه ها
Comparative Study of Chinese Hamster Ovary Cell Versus Escherichia coli-Derived Bone Morphogenetic Protein-2 Using the Critical-Size Supraalveolar Peri-Implant Defect Model
چکیده:   (1743 مشاهده)

  Background: Chinese hamster ovary (CHO) cell-derived recombinant human bone morphogenetic protein-2 (rhBMP-2) has been introduced for spine, long bone, and craniofacial indications. Escherichia coli- (E. coli) derived rhBMP-2 displays comparable efficacy to CHO cell-derived rhBMP-2 in vitro and in small-animal models. The objective of this study is to evaluate the efficacy of E. coli-derived rhBMP-2 compared to the benchmark CHO cell-derived rhBMP-2 using an established large-animal model.

 Methods: Contralateral, critical-size supraalveolar peri-implant defects in six adult male Hound Labrador mongrel dogs received CHO cell- or E. coli-derived rhBMP-2 (0.2 mg/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titanium mesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation.

 Results: Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell- and E. coli-derived rhBMP-2 supported comparable bone formation (new bone area, 35.8 ± 3.6 versus 30.1 ± 2.2 mm2 bone density, 31.8% ± 1.6% versus 35.6% ± 2.5% and osseointegration, 32.9% ± 7.4% versus 33.7% ± 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments.

 Conclusion: CHO cell- and E. coli-derived rhBMP-2 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.


 Source: Journal of Periodentology

 Full Text

     
نوع مطالعه: پژوهشي | موضوع مقاله: پریودنتولوژی
دریافت: 1392/4/20 | پذیرش: 1392/4/20 | انتشار: 1392/4/20
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Lee J, Lee E N, Yoon J, Chung S, Prasad H, Suusin C et al . Comparative Study of Chinese Hamster Ovary Cell Versus Escherichia coli-Derived Bone Morphogenetic Protein-2 Using the Critical-Size Supraalveolar Peri-Implant Defect Model. 3 2012; 6 (6)
URL: http://idai.ir/article-1-1295-fa.html

Comparative Study of Chinese Hamster Ovary Cell Versus Escherichia coli-Derived Bone Morphogenetic Protein-2 Using the Critical-Size Supraalveolar Peri-Implant Defect Model. 1. 1390; 6 (6)

URL: http://idai.ir/article-1-1295-fa.html



بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
دوره 6، شماره 6 - ( پریودنتولوژی 1390 ) برگشت به فهرست نسخه ها
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

کلیه حقوق سایت متعلق به جامعه اسلامی دندانپزشکان ایران است. تماس با ما

Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4710