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:: Volume 6, Issue 6 (1-2012) ::
3 2012, 6(6): 0-0 Back to browse issues page
Specificity of Antimicrobial Peptide LL-37 to Neutralize Periodontopathogenic Lipopolysaccharide Activity in Human Oral Fibroblasts
Wiroj Suphasiriroj * , Masato Mikami , Hiromi Shimomura , Soh Sato
Department of Periodontology, The Nippon Dental University, School of Life Dentistry at Niigata, Niigata, Japan
Abstract:   (1737 Views)

 Background: The antimicrobial peptide LL-37 is known to have a potent lipopolysaccharide (LPS)-neutralizing activity in various cell types. Because of observed heterogeneity within periodontopathogenic LPS, the authors hypothesized that LL-37 had specificity to neutralize such LPS activity. The present study, therefore, aims to investigate the LPS-neutralizing activity of LL-37 to various periodontopathogenic LPS in interleukin-8 (IL-8) production after challenging them in human oral fibroblasts.

 Methods: Human periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) were cultured from biopsies of periodontal ligament and gingival tissues. After cell confluence in 24-well plates, LPS (10 μg/mL) from Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans were added with or without LL-37 (10 μg/mL). After 18 hours, the supernatant was collected and analyzed in IL-8 production by enzyme-linked immunosorbent assay.

 Results: All periodontopathogenic LPS statistically significantly induced IL-8 production in both PDLF and GF (P <0.01). After neutralization with LL-37, both PDLF and GF showed a statistically significant reduction in IL-8 production compared with LPS-treated groups without LL-37 (P <0.01), and the percentage of reduction in IL-8 production in PDLF appeared to be higher than in GF. In addition, the percentage of reduction in IL-8 production varied considerably according to each periodontopathogenic LPS.

 Conclusions: The antimicrobial peptide LL-37 had an ability to suppress periodontopathogenic LPS-induced IL-8 production in both PDLF and GF. Its LPS-neutralizing activity revealed specificity to periodontopathogenic LPS and seemed to be dependent on the heterogeneity within LPS between different genera.


 Source: Journal of Periodentology

 Full Text

Keywords: Cathelicidin antimicrobial peptide, fibroblasts, lipopolysaccharides
     
Type of Study: Research | Subject: Periodontology
Received: 2013/07/11 | Published: 2012/01/15
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Suphasiriroj W, Mikami M, Shimomura H, Sato S. Specificity of Antimicrobial Peptide LL-37 to Neutralize Periodontopathogenic Lipopolysaccharide Activity in Human Oral Fibroblasts. 3 2012; 6 (6)
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Volume 6, Issue 6 (1-2012) Back to browse issues page
جامعه اسلامی دندانپزشکان Islamic Dental Association of IRAN

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