en پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <p> <strong>Background:</strong>  The aim of this study was to verify the validity of IL-21 local suppression in submandibular glands of preventing the development of Sjögren’s syndrome in non-obese diabetic (NOD) mice and figure out the mechanism. </p><p> <strong>Methods:</strong>  IL-21 levels in submandibular glands were suppressed by ductal cannulation of IL-21 shRNA lentivirus. Then, saliva flow rates (SFR) and histopathologic changes of submandibular glands were measured to assess the severity of disease development. Real-time PCR, flow cytometry, and immunohistochemistry were used to detect the changes of T helper cells and related cytokines. </p><p> <strong>Results:</strong>  The reduction in SFRs in NOD mice was significantly alleviated from 9 to 17 weeks of age along with the suppression of IL-21 in submandibular glands. Lymphocytic infiltration was also milder than control NOD mice. Moreover, the lower level of IL-21 led to the down-regulation of follicular helper T (Tfh) cells. </p><p> <strong>Conclusions:</strong>  Local suppression of IL-21 in submandibular glands could retard the development of Sjögren’s syndrome in NOD mice. IL-21 might contribute to the development of B-cell disorder in Sjögren’s syndrome via Tfh cells pathway. </p><hr><p></p><p> <strong>Source: </strong>Journal of Oral Pathology & Medicine </p><p><a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0714.2012.01175.x/abstract"><font color="#0033ff"> Full Text</font></a> </p> follicular helper T cells;IL-21;saliva gland;Sjögren’s syndrome 0 0 http://idai.ir/browse.php?a_code=A-10-32-837&slc_lang=en&sid=1