fa A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <p> <strong>Objective</strong></p><p>  Ameloblastoma has a high risk of bone invasion and local recurrence. However, the mechanisms of bone invasion in ameloblastoma remain unclear. In this study, we established an experimental model for matrix metalloproteinase (MMP) induction and osteoclastogenesis using ameloblastoma-derived cells. </p><p> <strong>Study design</strong></p><p>  We established an ameloblastoma-derived cell line without viral genes and analyzed the expression of all Wnt and Frizzled members and MMPs by real-time reverse transcription-polymerase chain reaction, and analyzed the activity of MMP-2 and MMP-9 by the in-gel-gelatinase assay. <strong></strong></p><p> <strong>Results</strong></p><p>  AM-3, newly established ameloblastoma-derived cells retained the morphology of primary-cultured ameloblastoma cells. AM-3 cells overexpressed the messenger RNA of Wnt-5a, Frizzled-2, MMP-2, and MMP-9 and showed the potential of osteoclastogenesis. In addition, Wnt-3a-treatment induced expression and activation of MMP-9 in AM-3 cells. </p><p> <strong>Conclusions</strong></p><p>  Our study suggests that AM-3 cells retained the characteristics of ameloblastoma, without acquiring typical features of cancer cells. Furthermore, Wnt signaling induced MMP-9 in ameloblastoma cells. </p><hr><p></p><p> <strong>Source: </strong>Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology </p><p> <a href="http://www.oooojournal.net/article/S2212-4403(13)00141-7/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> 0 0 http://idai.ir/browse.php?a_code=A-10-32-1636&slc_lang=fa&sid=1 Toshiro Kibe 10031947532846007622 10031947532846007622 Yes Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Takao Fuchigami 10031947532846007623 10031947532846007623 No Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Michiko Kishida 10031947532846007624 10031947532846007624 No Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Mikio lijima 10031947532846007625 10031947532846007625 No Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Kiyohide Ishihata 10031947532846007626 10031947532846007626 No Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Hiroshi Hijoka 10031947532846007627 10031947532846007627 No Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Akihiko Miyawaki 10031947532846007628 10031947532846007628 No Department of Oral and Maxillofacial Surgery, School of Medicine, University of Occupational and Environmental Health, Japan Ichiro Semba 10031947532846007629 10031947532846007629 No Department of Oral Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Norifumin Nakamura 10031947532846007630 10031947532846007630 No Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Japan Shosei Kishida 10031947532846007631 10031947532846007631 No Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan