en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background:</strong> Nitric oxide (NO) could be a potential target for the development of new therapeutic approaches to the treatment of periodontal disease because this molecule plays a significant role in the tissue destruction observed in periodontitis. In this study, the authors investigate the effect of kaempferol on the production of NO by murine macrophage-like RAW264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease, and try to determine the underlying mechanisms of action. <strong></strong></p><p> <strong>Methods:</strong> NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Real-time polymerase chain reaction was performed to quantify inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) mRNA expression. iNOS and HO-1 protein expression and phosphorylation of c-Jun N-terminal kinase and p38 were characterized via immunoblot analysis. Reactive oxygen species (ROS) production was measured using the redox-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate. </p><p> <strong>Results</strong>: Kaempferol significantly inhibited NO production and expression of iNOS protein in P. intermedia LPS-stimulated RAW246.7 cells without affecting iNOS mRNA expression. Kaempferol upregulated HO-1 expression in LPS-activated cells. Inhibition of HO-1 activity by tin protoporphyrin IX (SnPP) abolished the suppressive effect of kaempferol on NO production. In addition, kaempferol significantly attenuated P. intermedia LPS-induced increase of intracellular ROS, and SnPP blocked this reduction. Treatment with antioxidants downregulated the production of LPS-induced NO.</p><p> <strong> Conclusions</strong>: Kaempferol inhibits NO production and iNOS protein expression in P. intermedia LPS-stimulated RAW264.7 cells at the translational level via HO-1-mediated ROS reduction and could be an efficient modulator of host response in the treatment of periodontal disease. </p><hr><p></p><p> <strong>Source: </strong>Journal of Periodentology </p><p> <a href="http://www.joponline.org/doi/abs/10.1902/jop.2012.120180" target="_blank"><font color="#0000ff">Full Text</font></a></p> Heme oxygenase-1, kaempferol, lipopolysaccharides, nitric oxide, Prevotella intermedia, reactive oxygen species 0 0 http://idai.ir/browse.php?a_code=A-10-32-1286&slc_lang=en&sid=1 In Soon Choi 10031947532846005673 10031947532846005673 No Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, Korea Eun-Young Choi 10031947532846005674 10031947532846005674 No Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, Korea Ji-Young Jin 10031947532846005675 10031947532846005675 No Department of Biological Science, College of Medical and Life Sciences, Silla University, Busan, Korea Hae Ryoun Park 10031947532846005676 10031947532846005676 No Department of Oral Pathology, School of Dentistry, Pusan National University, Yangsan, Gyeongsangnam-do, Korea Jeom-Il Choi 10031947532846005677 10031947532846005677 No Department of Periodontology, School of Dentistry, Pusan National University/Biomedical Research Institute, Pusan National University Hospital, Busan, Korea Sung-Jo Kim sungjokim@pusan.ac.kr 10031947532846005678 10031947532846005678 Yes Department of Periodontology, School of Dentistry, Pusan National University/Biomedical Research Institute, Pusan National University Hospital, Busan, Korea