en Gene expression, nanotubes, osteoblasts, titanium disks جراحی دهان، فک و صورت Oral and Maxillofacial Surgery پژوهشي Research <p> <strong>Background:</strong> Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by in vivo studies with animal models and clinical trials over a forty‑year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. </p><p> <strong>Materials and Methods</strong>: To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT‑ PCR. </p><p> <strong>Results</strong>: After 15 days, osteoblasts cultivated on titanium naotube showed the up‑regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down‑regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up‑regulated while COL3A1, COL1A1, ALPL and SPP1 were down‑regulated. </p><p> <strong>Conclusions:</strong> Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2. </p><hr><p></p><p> <strong>Source: </strong>Dental Research Journal</p><p><a href="http://drj.mui.ac.ir/index.php/drj/article/view/1342" target="_blank"><font color="#0000ff"> Full Text</font></a></p> Gene expression, nanotubes, osteoblasts, titanium disks 0 0 http://idai.ir/browse.php?a_code=A-10-32-1145&slc_lang=en&sid=1 Alfonso Pozio 10031947532846004965 10031947532846004965 No ENEA, IDROCOMB, C.R. Casaccia, Rome Annalisa Palmieri 10031947532846004966 10031947532846004966 No Department of Medical-Surgical Sciences of Communication and Behavior, Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara Ambra Girardi 10031947532846004967 10031947532846004967 No Histology, Embryology and Applied Biology, Centre of Molecular Genetics, CARISBO Foundation,University of Bologna, Bologna, Italy Francesca Cura 10031947532846004968 10031947532846004968 No Histology, Embryology and Applied Biology, Centre of Molecular Genetics, CARISBO Foundation,University of Bologna, Bologna, Italy Francesco Carinci crc@unife.it 10031947532846004969 10031947532846004969 Yes Department of Medical-Surgical Sciences of Communication and Behavior, Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara