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en Bone augmentation potential in rabbit calvaria and ex vivo cytotoxicity of four bone substituting materials پریودنتولوژی Periodontology پژوهشي Research Background and aims. Tricalcium Phosphate (TCP), Bovine-Derived Hydroxyapatite (BioOss™), Demineralised Freeze-dried Bone Allograft (DFDBA) and Calcium Sulphate (CaS) were compared in vitro for osteoblast cytotoxicity and in rabbit’s calvaria to measure the bone histopathologic response.Materials and methods. 34 critical size defects in the calvaria of 12 male Australian rabbits were randomly filled with the materials and 2 empty defects were used as controls. After one month, histologicalal evaluation was performed on the samples to record regenerated bone type and volume, material absorption and the amount of inflammation. Saos-2 cell line was exposed to the materials and the cell line vitality was tested with Methyl Tetrazolium Test (MTT) to determine material’s osteoblast cell cytotoxicity.Results. The type of regenerated bone did not show a significant difference between the groups (p=1.0) while the amount of bone inflammation was significantly different (p=0.021), where BioOss caused the least and DFDBA had the highest. Bone formation was also similar between the groups (p=0.428). DFDBA group showed the highest material absorption while TCP group had the lowest (p=0.028). DFDBA was associated with significantly higher Saos-2 cell line viability than TCP and BioOss that were significantly less cytotoxic comparing to Cas (p< 0.0001).Conclusion. DFDBA group had the highest amount of material absorption and was associated with more inflammation than other materials in the rabbit calvaria. BioOss exhibited lowest amount of inflammation and TCP had the lowest amount of material absorption. Results of cytotoxicity test might be affected by different solubility constants of the test materials.<div align="left"><hr></div>ْSource: Journal of Periodontology & Implant Dentistry<a href="http://dentistry.tbzmed.ac.ir/jpid/index.php/jpid/article/view/2011e8">Full text</a> 0 0 http://idai.ir/browse.php?a_code=A-10-32-85&slc_lang=en&sid=1 Farzin Ghanavati fghanavati@yahoo.com 1003194753284600334 1003194753284600334 Yes Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Gholam Ali Gholami 1003194753284600335 1003194753284600335 No Shaheed Beheshti University of Medical Sciences, Tehran, Iran Saeid Asgari 1003194753284600336 1003194753284600336 No Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Mohammad Jafar Eghbal 1003194753284600337 1003194753284600337 No Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran Hesam Rahimi 1003194753284600338 1003194753284600338 No Harvard School of Dental Medicine, Boston, MA, USA Milad Karamlou 1003194753284600339 1003194753284600339 No Shaheed Beheshti University of Medical Sciences, Tehran, Iran Mahmoud Tamizi 1003194753284600340 1003194753284600340 No Mashhad University of Medical Sciences, Mashhad, Iran Farzam Ghanavati 1003194753284600341 1003194753284600341 No