en پریودنتولوژی Periodontology پژوهشي Research <b> Objective</b>: The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues.<div><b> Materials and Methods:</b> The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman’s rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3.</div><div><b> Results:</b> In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found. </div><div>  <b>Conclusion:</b> The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss.</div><hr><p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><b>Source: </b>Journal of Dentistry of Tehran University of Medical Sciences<span style="font-size: 15.5pt line-height: 115% font-family: Verdana, sans-serif color: rgb(250, 250, 250) background-color: rgb(101, 111, 115) background-position: initial initial background-repeat: initial initial"><o:p /></span></p> <p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><a href="http://jdt.tums.ac.ir/index.php/jdt/article/view/443" target="_blank"><font color="#0033ff">Full Text</font></a></p> TH17 cell;TH2 cell; IL-17F protein; GATA3 factors; Periodontal Disease 0 0 http://idai.ir/browse.php?a_code=A-10-32-964&slc_lang=en&sid=1 Parichehr Behfarnia 10031947532846004192 10031947532846004192 No Periodontics Department, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran Reza Birang 10031947532846004193 10031947532846004193 No Periodontics Department, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran Seyed Sajad Pishva 10031947532846004194 10031947532846004194 No Periodontics Department, Dental School, Uromiye University of Medical Sciences, Urmia, Iran Mazdac Ghanjali Hakemi 10031947532846004195 10031947532846004195 No Immunology Department, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Mitra Mohammadi Khorasani m.mohamadikh@yahoo.com 10031947532846004196 10031947532846004196 Yes