en کودکان Pediatric Dentistry پژوهشي Research <p> <strong>Purpose:</strong> The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin. </p><p> <strong>Methods:</strong> S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose. Biofilm acidogenicity was estimated by medium pH (2x/day). After 4 days for dentin and 5 days for enamel, biofilms were collected to obtain biomass, viable bacteria, total protein, and intra- and extracellular polysaccharides. Final SH was measured on the slabs. Demineralization was calculated as percentage of SH loss (%SHL). Differences were determined by analysis of variance and Tukey's test (P<.05). </p><p> <strong>Results:</strong> Biofilms formed on both substrates under 10% sucrose showed higher acidogenicity, biomass, protein concentration, insoluble polysaccharides, and %SHL than those treated with milk or lactose (P<.05), which did not differ in any parameter (P>.05). Higher bacterial counts were obtained from biofilms treated with milk on enamel, but not on dentin. Dentin was significantly more demineralized by milk than enamel (P<.05). </p><p> <strong>Conclusions:</strong> Although bovine milk appears to be less cariogenic than sucrose, this dairy product should not be considered caries-safe. </p><hr><p></p><p> <strong>Source: </strong>Journal of American Academy of Pediatric Dentistry </p><p><a href="http://www.ingentaconnect.com/content/aapd/pd/2012/00000034/00000007/art00007" target="_blank"><font color="#0000ff"> Full Text</font></a> </p> 0 0 http://idai.ir/browse.php?a_code=A-10-32-930&slc_lang=en&sid=1 Cecilia Muñoz-Sandoval 10031947532846004055 10031947532846004055 No Dental School of the University of Talca, Specialization Program in Pediatric Dentistry, the University of Talca, Talca, Chile María José Muñoz-Cifuentes 10031947532846004056 10031947532846004056 No Dental School of the University of Talca, the University of Talca, Talca, Chile Rodrigo A Giacaman giacaman@utalca.cl 10031947532846004057 10031947532846004057 Yes Cariology Unit, Department of Oral Rehabilitation, the University of Talca, Talca, Chile Renzo A. Ccahuana-Vasquez 10031947532846004058 10031947532846004058 No Braun - Procter & Gamble, Kronberg, Germany Jaime A. Cury 10031947532846004059 10031947532846004059 No Piracicaba Dental School, UNICAMP, Piracicaba, São Paulo, Brazil