en بیماری های دهان Oral Medicine پژوهشي Research <p> <strong>Objectives</strong> MicroRNAs (miRNAs) are endogenous small non-coding RNAs that negatively regular target gene expression by RNA interference. The processing of the pre-miRNA hairpin generates a miRNA duplex, which consists of a miRNA (guide strand) and a miRNA (passenger strand). miR-31 is an oncogenic miRNA and is up-regulated in oral squamous cell carcinoma (OSCC). miR-31 shows a high level of conservation across species and, based on this, this study hypothesized that miR-31 is a functional miRNA. </p><p> <strong>Materials and Methods</strong> The expression of miR-31 and miR-31 in OSCC tissues and oral cells were analyzed. Functional studies were performed on OSCC cells. </p><p> <strong>Results</strong> miR-31 is up-regulated in OSCC tissues, but its expression is less abundant than miR-31. miR-31 decreases the proliferation and migration of both SAS and Fadu cells. Furthermore, miR-31 targets the 3′UTR of RhoA and is able to down-regulate RhoA expression. Knockdown of RhoA expression is known to decrease the proliferation and migration of OSCC cells. However, up-regulation of both miR-31 and miR-31 by delivery of pre-mir-31 does still enhance OSCC oncogenicity. </p><p> <strong>Conclusion</strong> miR-31 is a functional miRNA involving in regulating RhoA, and the activity of miR-31’s activity seems to counteract the functions of miR-31 during OSCC tumorigenesis. </p><hr><p></p><p> <strong>Source: </strong>Journal of Oral Oncology </p><p><a href="http://www.oraloncology.com/article/S1368-8375(12)00231-X/abstract"><font color="#0000ff"> Full Text</font></a> </p> Carcinoma, Cell movement, Cell proliferation, MicroRNAs, Mouth, RNA interference 0 0 http://idai.ir/browse.php?a_code=A-10-32-851&slc_lang=en&sid=1 Kuo-Wei Chang 10031947532846003693 10031947532846003693 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University,Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan, Shou-Yen Kao 10031947532846003694 10031947532846003694 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University,Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan, Yi-Hsuan Wu 10031947532846003695 10031947532846003695 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan Meng-Miao Tsai 10031947532846003696 10031947532846003696 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan Hsi-Feng Tu 10031947532846003697 10031947532846003697 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan Chung-Ji Liu 10031947532846003698 10031947532846003698 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan,Department of Oral and Maxillofacial Surgery, Taipei MacKay Memorial Hospital, Taipei, Taiwan Mann-Tin Lui 10031947532846003699 10031947532846003699 No Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan,Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan Shu-Chun Lin 10031947532846003700 10031947532846003700 Yes Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan