en پریودنتولوژی Periodontology پژوهشي Research <p> Background: Many studies have shown favorable results following the use of different bone graft<br>materials. The aim of the present study was to evaluate the biocompatibility of four different bone<br>graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro.<br>Materials and Methods: The effects of Bio-Oss®, Tutodent®, Osteon®, and Cerasorb® were<br>studied on the human osteoblast-like cell line to evaluate various parameters. Human osteoblastlike<br>cells were seeded onto the mentioned bone substitute materials (BSMs). Cell differentiation<br>cell viability and alkaline phosphatase (ALP) activity of the seeded cells were evaluated by means of<br>scanning electron microscopy, cell viability test and phase contrast microscopy Analysis of variance<br>(ANOVA). Tamhane´s post-hoc, Kruskal-Wallis Test, and Dunn’s Test were used. The results were<br>considered to be statistically significant at P<0.05.<br>Results: The control group (SaOS-2 cells which were incubated in Dulbecco Modified Eagle Medium<br>without any kind of bone graft materials) had the highest level of cell viability (P<0.001), followed<br>by Tutodent®, Osteon®, Cerasorb®, and Bio-Oss®. There was no significant difference in MTT assay<br>results between Tutodent® and the control group (P=0.032). All tested bone graft materials showed<br>significantly higher ALP activity than the control (P<0.001). The Tutodent® group showed the best<br>cell growth among all experimental groups, followed by the Osteon® group. The former had a higher<br>spindle-like morphology with good attachment to the surface. Cells cultivated on the surfaces of<br>the Cerasorb® and Bio-Oss® granules had more round morphologies.<br>Conclusion: This in vitro study demonstrated that all tested BSMs can provide good cell<br>differentiation but a lower rate of proliferation.</p><hr><p></p><p> <strong>Source:</strong> Dental Research Journal</p><p><a href="http://www.drj.mui.ac.ir/index.php/drj/article/view/901"><font color="#0000ff"> Full text</font></a> </p> Alkaline phosphatase, bone graft, cell culture, cell morphology, cell viability, osteoblast 0 0 http://idai.ir/browse.php?a_code=A-10-32-385&slc_lang=en&sid=1 Nader Ayobian-Markazi na1344@yahoo.com 10031947532846001619 10031947532846001619 Yes Department of Periodontics, Dental Branch, Islamic Azad University, Tehran, Iran T. Fourootan 10031947532846001620 10031947532846001620 No Department of Biology, Tarbiat Modrres University, Tehran, Iran M. J. Kharazifar 10031947532846001621 10031947532846001621 No Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran