en پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <div class="svAbstract"><div class="articleText"><div class="articleText_indent"><h3 class="h3">Summary</h3>The development of locoregional recurrence is the main reason for treatment failure in head and neck squamous cell carcinomas (HNSCC) and the remaining of tumor cells in surgical margins is associated with recurrence. Surgical margins are considered negative based on histologic assessment of the pathological specimen. However, this method lacks sensitivity in identifying cells that already started malignant transformation but have not yet developed a pathologic phenotype. We investigated the usefulness of assessing the expression of <em>PTHLH</em>, <em>EPCAM</em>, <em>MMP9</em>, <em>LGALS1</em> and <em>MET</em> for the detection of molecular alterations in histologically negative surgical margins and determine the correlation of these tumor-related alterations with clinical and prognostic parameters. Differential gene expression was determined by quantitative RT–PCR analyses in normal mucosa, HNSCC and negative margin samples. Thirty-eight percent of the histologically negative surgical margins examined were margin-positive for overexpression of at least one of the genes evaluated. Moreover, <em>MMP9</em> and <em>PTHLH</em> overexpression in the surgical margins was associated with the development of second primary tumors (<em>p</em> = 0.002) and lower rates of local control (log rank test <em>p</em> = 0.022 <em>HR</em> = 4.186 <em>p</em> = 0.035), respectively. These findings demonstrate that the overexpression of tumor-related genes in histologically negative surgical margins is a frequent event. The use of qRT–PCR may be an useful tool in detecting actually negative HNSCC surgical margins and the overexpression of specific genes in these margins could be helpful in the identification of patients with a higher risk of developing second primary tumors and local recurrences, thus aiding the surgeon in the delineation of the HNSCC resection extent and helping in the planning of adjuvant therapy.</div><div class="articleText_indent"><hr></div><div class="articleText_indent"><font size="3"><p dir="ltr"><strong>Source:</strong> Journal of Oral Oncology</p><p dir="ltr"><a href="http://www.sciencedirect.com/science/article/pii/S1368837511008785"><font color="#0000cc">Full text</font></a></p></font></div><div class="articleText_indent"></div></div></div> 0 0 http://idai.ir/browse.php?a_code=A-10-32-323&slc_lang=en&sid=1 Ana Carolina de Carvalho 10031947532846001391 10031947532846001391 Yes Luiz Paulo Kowalski 10031947532846001392 10031947532846001392 No Antônio Hugo 10031947532846001393 10031947532846001393 No José Fróes Marques Campos 10031947532846001394 10031947532846001394 No Fernando Augusto Soares 10031947532846001395 10031947532846001395 No André Lopes Carvalho 10031947532846001396 10031947532846001396 No André Luiz Vettore 10031947532846001397 10031947532846001397 No