fa Incidence assessment of MTHFR C677T and A1298C polymorphisms in Iranian non-syndromic cleft lip and/or palate patients کودکان Pediatric Dentistry پژوهشي Research <b> Background and aims</b>. The aim of the present study is to determine the incidence of MTHFR C677 T and A1298C mutations in Iranian patients with cleft lip and/or cleft palate. <div><b>Materials and methods</b>. We screened 61 Iranian patients with cleft lip and/or cleft palate for mutations in the two alleles of MTHFR gene associated with cleft lip and/or palate: A1298C and C677T, using Polymerase Chain Reaction following by RFLP. <div><b>Results.</b> The 677T and 1298C homozygote genotypes showed a frequency of 36.1% and 11.4%, respectively. Combined genotype frequencies in newborns having oral clefts showed that the highest genotype was 677TT/1298AA (22.9%) and 677TT/1298CC genotypes were not observed. </div><div><b>Conclusion.</b> The results showed that 65.6% of all patients had at least one T mutant allele in C677T and 58.9% C mutant allele for A1298C. According to the frequencies of homozygosity of mutant alleles, it could be said that MTHFR genotype of 677TT shows a greater role in having oral clefts.</div><hr><p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><b>Source: </b>Journal of Dental Research, Dental Clinics, Dental Prospects </p> <p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><a href="http://dentistry.tbzmed.ac.ir/joddd/index.php/joddd/article/view/1685" target="_blank"><font color="#0000ff">Full Text</font></a></p></div> A1298C, C677T, methylenetetrahydrofolate reductase, orofacial cleft, polymorphism 0 0 http://idai.ir/browse.php?a_code=A-10-32-3323&slc_lang=fa&sid=1 Asghar Ebadifar ebadifar@hbi.ir 100319475328460015303 100319475328460015303 Yes Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Behehsti University of Medical Sciences, Tehran, Iran Nazila Ameli 100319475328460015304 100319475328460015304 No Dental School, Semnan University of Medical Sciences, Semnan, Iran Hamidreza KhorramKhorshid 100319475328460015305 100319475328460015305 No Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Mehdi Salehi zienabadi 100319475328460015306 100319475328460015306 No Dental School, Semnan university of Medical Sciences, Semnan, Iran Kourosh Kamali 100319475328460015307 100319475328460015307 No Avicenna Research Institute, ACECR, Tehran, Iran Tayyebe Khoshbakht 100319475328460015308 100319475328460015308 No Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran