en پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <div class="para"><p><b>Background: </b> Loco-regional spread of disease causes high morbidity and is associated with the poor prognosis of malignant oral tumors. Better understanding of mechanisms underlying the establishment of lymph node metastasis is necessary for the development of more effective therapies for patients with oral cancer. The aims of this work were to evaluate a possible correlation between endothelial cell Bcl-2 and lymph node metastasis in patients with oral squamous cell carcinoma (OSCC), and to study signaling pathways that regulate Bcl-2 expression in lymphatic endothelial cells.</p></div><div class="para"><p><b>Methods: </b> Endothelial cells were selectively retrieved from paraffin-embedded tissue sections of primary human OSCC from patients with or without lymph node metastasis by laser capture microdissection. RT-PCR was used to evaluate Bcl-2 expression in tumor-associated endothelial cells and in tumor cells. <em>In vitro</em>, mechanistic studies were performed to examine the effect of vascular endothelial growth factor (VEGF)-C on the expression of Bcl-2 in primary human lymphatic endothelial cells.</p></div><div class="para"><p><b>Results: </b> We observed that Bcl-2 expression is upregulated in the endothelial cells of human oral tumors with lymph node metastasis as compared to endothelial cells from stage-matched tumors without metastasis. VEGF-C induced Bcl-2 expression in lymphatic endothelial cells via VEGFR-3 and PI3k/Akt signaling. Notably, OSCC cells express VEGF-C and induce Bcl-2 in lymphatic endothelial cells.</p></div><div class="para"><p><b>Conclusions: </b> Collectively, this work unveiled a mechanism for the induction of Bcl-2 in lymphatic endothelial cells and suggested that endothelial cell Bcl-2 contributes to lymph node metastasis in patients with oral squamous cell carcinoma.</p><p><hr></p><font size="3"><p dir="ltr"><strong>Source:</strong> Journal of Oral Pathology & Medicine</p><p dir="ltr"><a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0714.2011.01081.x/abstract" target="_blank"><font color="#0000cc">Full text</font></a></p></font></div> angiogenesis,biomarkers,head and neck cancer,lymphangiogenesis,oral cancer,vascular endothelial growth factor 0 0 http://idai.ir/browse.php?a_code=A-10-32-302&slc_lang=en&sid=1 Sandra B. C. Tarquinio 10031947532846001256 10031947532846001256 Yes Zhaocheng Zhang 10031947532846001257 10031947532846001257 No Kathleen G. Neiva 10031947532846001258 10031947532846001258 No Peter J. Polverini 10031947532846001259 10031947532846001259 No Jasques E. Nör 10031947532846001260 10031947532846001260 No