en پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <div class="para"><p><b>Objective: </b> ‘Field cancerization’ is an accepted model for oral carcinogenesis. So far, genetically altered fields have been just reported in the presence of carcinomas. This study assessed the distant mirror fields (MFs) of oral precancer by DNA high-resolution flow cytometry (hr DNA-FCM) and array-Comparative Genomic Hybridization (a-CGH).</p></div><div class="para"><p><b>Methods: </b> Five leukoplakias without dysplasia (OLs), seven dysplastic leukoplakias (DOLs), and 12 corresponding visually normal and non-dysplastic MFs were analyzed. DNA aneuploidy (DNA Index, DI ≠ 1) was detected by hr DNA-FCM on DAPI stained nuclei suspensions. The epithelial DNA aneuploid subclones were FCM-sorted to obtain genomic DNA for a-CGH.</p></div><div class="para"><p><b>Results: </b> Mirror fields, OLs, and DOLs showed increasing prevalence of DNA aneuploidy of, respectively, 8%, 20%, and 57%. The average number of chromosome aberrations (Ch-Abs) was 2.8 in MFs, 3 in OLs, and 10.6 in DOLs. MFs relative to OLs and DOLs had average numbers of Ch-Abs, respectively, of 1.8 and 3.6. Ch-Abs were also observed in DNA diploid sublines, and often the same aberrations were observed in both MFs and corresponding OLs/DOLs.</p></div><div class="para"><p><b>Conclusion: </b> DNA aneuploidy and Ch-Abs in MFs, the last ones being mainly gains, indicate an early onset of field effect in oral carcinogenesis.</p><p><hr></p><font size="3"><p dir="ltr"><strong>Source:</strong> Hournal of Oral Pathology & Medicine</p><p dir="ltr"><a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0714.2011.01085.x/abstract" target="_blank"><font color="#0000cc">Full text</font></a></p></font></div> field cancerization,high-resolution DNA flow cytometry,high-resolution oligonucleotide array-comparative genomic hybridization,oral precancer 0 0 http://idai.ir/browse.php?a_code=A-10-32-301&slc_lang=en&sid=1 Monica Pentenero 10031947532846001247 10031947532846001247 Yes Alessandra Donadini 10031947532846001248 10031947532846001248 No Emanuela Di Nallo 10031947532846001249 10031947532846001249 No Massimo Maffei 10031947532846001250 10031947532846001250 No Roberto Marino 10031947532846001251 10031947532846001251 No Ubaldo Familiari 10031947532846001252 10031947532846001252 No Patrizio Castagnola 10031947532846001253 10031947532846001253 No Sergio Gandolfo 10031947532846001254 10031947532846001254 No Walter Giaretti 10031947532846001255 10031947532846001255 No