en پریودنتولوژی Periodontology پژوهشي Research <p> Purpose: The purpose of this study was to exploit potential methods of surface modification for improving the seal between the neck portion of a dental implant and the surrounding soft tissue. <br>Materials and Methods: Titanium surfaces were modified by machining (SM-Ti group) machining and acid etching (AE-Ti group) or machining, acid etching, and depositing 4.5 collagen/hyaluronic acid (col/HA) polyelectrolyte bilayers (CHC-Ti group). These were analyzed using scanning electron microscopy, scanning force microscopy, x-ray photoelectron spectroscopy, contact angle measurement, and quartz crystal microbalance measurement. The degradation behavior of the col/HA multilayer coating was measured. Next, human gingival fibroblasts (HGFs) were cultured on the different surfaces, and cell morphology and spreading were observed using fluorescence microscopy and a shape factor measurement. Cell proliferation was examined by fluorometric quantification of the amount of cellular DNA. Matrix formation of HGFs was determined via enzyme-linked immunosorbent assay. Gene expression was analyzed via reverse transcriptase polymerase chain reaction. <br>Results: Similar surface topology for these three groups was observable on a microscopic scale, and morphologic differences were apparent on the nanoscale. Both acid etching and col/HA deposition improved the hydrophilicity of the titanium surface, in contrast to machining alone. Each col/HA bilayer was about 5 nm thick. The col/HA coating degraded in about a week. Attachment and spreading of HGFs was better on the CHC-Ti surface than on the SM-Ti or AE-Ti surfaces. Moreover, the proliferation and differentiation of HGFs were greatly stimulated when cultured on CHC-Ti. <br>Conclusion: In contrast to two control surfaces (one machined, one machined and acid-etched), col/HA treatment of Ti improved the attachment, spreading, proliferation, and differentiation of HGFs.<br></p><hr><p></p><p> <strong> Source </strong>: Journal of Oral & Maxillofacial Implant </p><p><a href="http://jomi.quintessenz.de/index.php?jid=jomi&doc=toc&year=2011&issue=6" target="_blank"><font color="#0000cc">  Full text </font></a></p> coating, dental implant, implant neck, soft tissue integration, surface properties 0 0 http://idai.ir/browse.php?a_code=A-10-32-280&slc_lang=en&sid=1 Feng Zhang 10031947532846001144 10031947532846001144 Yes Ying Huang 10031947532846001145 10031947532846001145 No Xiaodong Li 10031947532846001146 10031947532846001146 No Shifang Zhao 10031947532846001147 10031947532846001147 No