fa Antibacterial effect of morphous (poly-crystalline) and amorphous (glass) nano-bioactive glass 45S5 on Streptococcus mutans جراحی دهان، فک و صورت Oral and Maxillofacial Surgery پژوهشي Research <p style="background-color: rgb(239, 243, 250)"><strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px">Objective: </i></strong>Bioactive glass 45S5 is a surface reactive glass-ceramic biomaterial, developed in 1969. BAG 45S5 with particle size of 20-60 nm has the ability of bone regeneration, broad spectrum antibacterial effect, repairs and replaces diseased or damaged bone. The aim of this study was to evaluate the antibacterial activity and determine MIC and MBC values of nano-BAG45S5 on <i style="margin: 0px padding: 0px border: 0px">Streptococcus mutans</i>.</p><p style="background-color: rgb(239, 243, 250)"> <strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px"> Methods: </i></strong>In this study the <i style="margin: 0px padding: 0px border: 0px">in vitro</i> Antibacterial activity of polycrystalline and glass forms of nano-BAG 45S5 was evaluated. Bacterial susceptibility to test materials was examined by antibiogram test. Afterwards MIC and MBC assays were conducted via broth dilution, disc diffusion and colony count methods.</p><p style="background-color: rgb(239, 243, 250)"> <strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px"> Results: </i></strong>Despite amorphous nano-BAG 45S5 , poly-crystalline form had antibiogram negative test result. In broth dilution test, the optical absorbance of test dilution of 50mcg/ml and higher concentrations were equal to negative control’s optical absorbance and their inhibitory zone diameter were measured 10.0mm in disc diffusion test. No colony was observed on the culture media of test dilution of 200mcg/ml and higher concentrations.</p><strong style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)"><i style="margin: 0px padding: 0px border: 0px">Conclusion: </i></strong><i style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">Streptococcus mutans </i><span style="font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">(ATCC 35668) is not susceptible to poly-crystalline</span><strong style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)"> </strong><span style="font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">nano -BAG45S5 . Amorphous</span><strong style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)"> </strong><span style="font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">nano-BAG45S5 is bacteriostatic against </span><i style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">Streptococcus mutans</i><span style="font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">. MIC and</span><strong style="margin: 0px padding: 0px border: 0px font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)"> </strong><span style="font-size: 11px line-height: 24.2000007629395px background-color: rgb(239, 243, 250)">MBC values for amorphous nano-BAG45S5 were 50 ppm and 200 ppm, respectively.</span><hr><p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><b>Source: </b>Journal of Dental School Shahid Beheshti University of Medical Sciences </p> <p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><a href="http://jds.sbmu.ac.ir/browse.php?a_id=1407&sid=1&slc_lang=en" target="_blank"><font color="#0000ff">Full Text</font></a></p> MBC, MIC, Morphology, Nano-BAG45S5, Streptococcus mutans, 0 0 http://idai.ir/browse.php?a_code=A-10-32-2956&slc_lang=fa&sid=1 Hossein Shahoon 100319475328460013742 100319475328460013742 No Dept. of Oral & Maxillofacial Surgery, School of Dentistry, Shahed University, Tehran, Iran. Mohammad Niyakan 100319475328460013743 100319475328460013743 No Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran. Mona Behnami 100319475328460013744 100319475328460013744 No School of Dentistry, Shahed University, Tehran, Iran Masoumeh Behnami mmbhn83@yahoo.com 100319475328460013745 100319475328460013745 Yes Sara Shahbazi 100319475328460013746 100319475328460013746 No Dept. of biomaterials, Medical Engineering Faculty, Amir Kabir University, Tehran, Iran