fa Micronucleus assay- an early diagnostic tool to assess genotoxic changes in tobacco and related habits بیماری های دهان Oral Medicine پژوهشي Research <p style="background-color: rgb(239, 243, 250)"><strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px">Objective: </i></strong>Micronuclei are induced in cells by a variety of substances, like UV radiation, infrared rays, X-radiations, and chemicals. Among them tobacco- specific nitrosamines have been reported to be potent mutagenic agents which are thought to be responsible for the induction of chromosomal aberrations resulting in production of micronuclei. The main aim of our study is to compare MN frequency among subjects, chewing tobacco only, chewing and smoking tobacco only, and chewing, smoking with alcohol, and to co-relate with control subjects.</p><p style="background-color: rgb(239, 243, 250)"> <strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px"> Methods: </i></strong>Healthy subjects are included in the study and divided into four groups having 20 subjects in each group. Group-I is chewing only, group-II chewing and smoking, group-III chewing and smoking with alcohol, group-IV control. Smears were made from buccal exfoliated cells and stained with DNA specific stain Acridine orange. Frequency on MNC per 100 cells was assessed with , one way ANOVA & Tukey HSD Multiple Comparisons test with P<0.05.</p><p style="background-color: rgb(239, 243, 250)"> <strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px"> Results: </i></strong>The mean number of MN was 2.3, 2.4, 3.6 in the group of chewing only, chewing & smoking, chewing, smoking & alcohol respectively. While assessing MN in the controls, out of 20 cases, 19 showed no MN among the cells examined while 1 patient showed 1 MN each per 100 cells examined.</p><p style="background-color: rgb(239, 243, 250)"> <strong style="margin: 0px padding: 0px border: 0px"><i style="margin: 0px padding: 0px border: 0px"> Conclusion: </i></strong>The present study concludes that MN is a better surrogate biomarker to predict genotoxicity for tobacco related habits.</p><hr><p class="MsoNormal" dir="rtl"><br></p> <p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><b>Source: </b>Journal of Dental School Shahid Beheshti University of Medical Sciences </p> <p class="MsoNormal" style="text-align: left direction: ltr unicode-bidi: embed"><a href="http://jds.sbmu.ac.ir/browse.php?a_id=1343&sid=1&slc_lang=en" target="_blank">Full Text</a></p> Micronuclei, tobacco, smoking, alcohol, genotoxic, Acridine orange 0 0 http://idai.ir/browse.php?a_code=A-10-32-2932&slc_lang=fa&sid=1 Smit Singla smitcool2@yahoo.co.in 100319475328460013636 100319475328460013636 Yes Dept. of Oral Medicine & Radiology, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, India Vathsala Naik 100319475328460013637 100319475328460013637 No Dept. of Oral Medicine & Radiology, A.J. Institute of Dental Sciences, Mangalore, India Raghavendra Kini 100319475328460013638 100319475328460013638 No Dept. of Oral Medicine & Radiology, A.J. Institute of Dental Sciences, Mangalore, India Anjali Shetty 100319475328460013639 100319475328460013639 No Dept. of Oral Medicine & Radiology, A.J. Institute of Dental Sciences, Mangalore, India