en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background:</strong> In the present study, the expression and localization of three epithelial peptides (human β-defensin [hBD]-2 and -3, and cathelicidin [LL-37]) are studied in an organotypic dento-epithelial (OD-E) model exposed to <i>Fusobacterium nucleatum</i> (<i>Fn</i>) biofilm.</p><p> <strong>Methods:</strong> Biofilm of <i>Fn</i> ATCC 25586 or AHN 9508 were produced by culturing each strain on semipermeable membranes. The OD-E model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pieces on the top. A 3-day-old biofilm was placed on the top of the OD-E and the coculture was incubated for 5 hours or 24 hours. Production of epithelial antimicrobial peptides was determined immunohistochemically.</p><p> <strong>Results:</strong> After 5 hours of incubation, the biofilm of each <i>Fn</i> strain stimulated expression of hBD-2 and -3. hBD-2 was localized on superficial layers and hBD-3 on basal cell layers of the epithelium and dento-epithelial junctions, whereas LL-37 was only weakly expressed. After 24 hours, hBD-2 expression was extended toward basal cell layers of the epithelium. In contrast, hBD-3 expression extended toward superficial layers of the epithelium. In the case of <i>Fn</i> AHN 9508 biofilm, LL-37 was localized in the cell layers of the dento-epithelial junction.</p><p> <strong>Conclusion:</strong> In our OD-E model, epithelial antimicrobial peptide responses to <i>Fn</i> biofilms have distinct regulation and localization characteristics, resembling those known to occur in the gingival epithelium in vivo. </p><hr><p></p><p> <strong> Source </strong>: Journal of Periodentology </p><p><a href="http://www.joponline.org/doi/abs/10.1902/jop.2011.110177" target="_blank"><font color="#0000cc">  Full text </font></a></p> Beta-defensin 2, human, beta-defensin 3, human, epithelium, Fusobacterium nucleatum, LL-37 peptide 0 0 http://idai.ir/browse.php?a_code=A-10-32-275&slc_lang=en&sid=1 Ulvi K. Gursoy ulvi.gursoy@utu.fi 10031947532846001118 10031947532846001118 Yes Institute of Dentistry, University of Turku, Turku, Finland Marja Pöllänen 10031947532846001119 10031947532846001119 No Institute of Dentistry, University of Turku, Turku, Finland Eija Könönen 10031947532846001120 10031947532846001120 No National Institute for Health and Welfare, Helsinki, Finland Veli-Jukka Uitto 10031947532846001121 10031947532846001121 No Institute of Dentistry, University of Helsinki, Helsinki University Central Hospital, Helsinki, Finland