fa DNA Damage in Oral Mucosa Cells of Patients with Fixed Orthodontic Appliances ارتودنسی Orthodontics پژوهشي Research <p> <strong>Objective:</strong></p><p>  The release of toxic metal ions from orthodontic alloys has induced concerns regarding the biocompatibility of fixed appliances. This study investigated the genotoxic effect of metal appliances in a sample of patients undergoing fixed orthodontic treatment. </p><p> <strong>Materials and Methods:</strong></p><p>  The study included twenty-five healthy individuals requiring orthodontic therapy in both jaws. The patients were treated by stainless steel orthodontic brackets and nickel-titanium or stainless steel arch wires. The oral mucosa cells were gathered just before the appliance placement and 9 months later. The cells were centrifuged, fixed and dropped onto slides. After staining, the micronucleus (MN) assay was used to determine genome alteration. The data were analyzed by paired sample t-test. </p><p> <strong>Results:</strong></p><p>  The mean micronuclei frequency in the buccal mucosa was 10.6 ± 5.7 per 1000 cells before the appliance placement and 9.2 ± 6.37 per 1000 cells 9 months later. No significant difference was found in the MN count before and 9 months after therapy (p=0.336).</p><p> <strong> Conclusion:</strong> </p><p> Under the conditions used in this study, application of fixed orthodontic appliances did not expose healthy individuals to increased risk of DNA damage in oral mucosa cells. </p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry of Tehran University of Medical Science</p><p> <a href="http://jdt.tums.ac.ir/index.php/jdt/article/view/1067.pdf" target="_blank"><font color="#0000ff">Full Text</font></a></p> Orthodontic Appliances; DNA Damage; Micronucleus Test; Biocompatibility; Genotoxicity 0 0 http://idai.ir/browse.php?a_code=A-10-32-2662&slc_lang=fa&sid=1 Farzin Heravi 100319475328460012534 100319475328460012534 No Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran Mohammad Reza Abbaszadegan 100319475328460012535 100319475328460012535 No Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran Mohsen Merati 100319475328460012536 100319475328460012536 No Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran Nadia Hasanzadeh 100319475328460012537 100319475328460012537 No Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran Ezzat Dadkhah 100319475328460012538 100319475328460012538 No Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran Farzaneh Ahrari farzaneh.ahrari@gmail.com 100319475328460012539 100319475328460012539 Yes Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran