fa DSG3 as a biomarker for the ultrasensitive detection of occult lymph node metastasis in oral cancer using nanostructured immunoarrays بیماری های دهان Oral Medicine پژوهشي Research <p> <strong>Objectives </strong></p><p> The diagnosis of cervical lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) patients constitutes an essential requirement for clinical staging and treatment selection. However, clinical assessment by physical examination and different imaging modalities, as well as by histological examination of routine lymph node cryosections can miss micrometastases, while false positives may lead to unnecessary elective lymph node neck resections. Here, we explored the feasibility of developing a sensitive assay system for desmoglein 3 (DSG3) as a predictive biomarker for lymph node metastasis in HNSCC. </p><p> <strong>Materials and methods</strong> </p><p> DSG3 expression was determined in multiple general cancer- and HNSCC-tissue microarrays (TMAs), in negative and positive HNSCC metastatic cervical lymph nodes, and in a variety of HNSCC and control cell lines. A nanostructured immunoarray system was developed for the ultrasensitive detection of DSG3 in lymph node tissue lysates. </p><p> <strong>Results</strong></p><p>  We demonstrate that DSG3 is highly expressed in all HNSCC lesions and their metastatic cervical lymph nodes, but absent in non-invaded lymph nodes. We show that DSG3 can be rapidly detected with high sensitivity using a simple microfluidic immunoarray platform, even in human tissue sections including very few HNSCC invading cells, hence distinguishing between positive and negative lymph nodes. <strong></strong></p><p> <strong>Conclusion</strong></p><p>  We provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis and guide in the selection of appropriate therapeutic intervention modalities for HNSCC patients. </p><hr><p></p><p> <strong>Source: </strong>Journal of Oral Oncology</p><p> <a href="http://www.oraloncology.com/article/S1368-8375(12)00261-8/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> DSG3, Head and neck cancer, Desmosomes, Biomarker, Sentinel lymph nodes, Nanosensors, Immunoarray, Lymph nodes metastasis, Proteomics, SCC 0 0 http://idai.ir/browse.php?a_code=A-10-32-2432&slc_lang=fa&sid=1 Vyomesh Patel 100319475328460011514 100319475328460011514 No Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health Daniel Martin 100319475328460011515 100319475328460011515 No Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health Malhotra Ruchika 100319475328460011516 100319475328460011516 No Departments of Chemistry and Cell Biology, University of Connecticut, Storrs, CT, United States Christina A. Marsh 100319475328460011517 100319475328460011517 No Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda Colleen L. Doçi 100319475328460011518 100319475328460011518 No Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda Timothy D. Veenstra 100319475328460011519 100319475328460011519 No Laboratory of Proteomics and Analytical Technologies, Science Applications International Corporation-Frederick, Inc., National Cancer Institute, Frederick, MD, United States Cherie-Ann O. Nathan 100319475328460011520 100319475328460011520 No Department of Otolaryngology, Head and Neck Surgery, Louisiana State University Health Sciences Center, Shreveport, LA, United States Uttam K. Sinha 100319475328460011521 100319475328460011521 No Department of Otolaryngology, Head and Neck Surgery, University of Southern California, Keck School of Medicine, Los Angeles, CA, United States Bhuvanesh Singh 100319475328460011522 100319475328460011522 No Laboratory of Epithelial Cancer Biology, Head and Neck Service, Memorial Sloan-Kettering Cancer Center, New York J. Silvio Gutkind 100319475328460011523 100319475328460011523 Yes Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health