fa Expression of Bak and Bak/Mcl-1 ratio can predict photodynamic therapy outcome for oral verrucous hyperplasia and leukoplakia بیماری های دهان Oral Medicine پژوهشي Research <p> <strong>Background </strong></p><p> Our previous studies showed that topical 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is very effective for oral verrucous hyperplasia (OVH) and relatively less effective for oral leukoplakia (OL) lesions. Nevertheless, there has been no report on the association of the expression of apoptosis-related proteins in OVH and OL biopsy tissues prior to PDT with PDT treatment outcomes.</p><p> <strong> Methods</strong> </p><p> This study used immunohistochemistry to evaluate whether the expression of Bak, Mcl-1, caspase-3, caspase-8, caspase-9, p53, p21, or PCNA protein in biopsy specimens of OVH and OL lesions could be used to predict the clinical outcomes of 18 OVH and 40 OL lesions treated with topical ALA-PDT. The marker labeling score (LS) was defined as labeling index (positive cells/total cells) multiplied by staining intensity. The lesions after ALA-PDT treatment were divided into complete response (CR) group and partial or no response (PR/NR) group. </p><p> <strong>Results</strong></p><p>  The mean Bak LS and the mean Bak/Mcl-1 LS ratio were significantly higher in the CR group than in the PR/NR group. However, there was no significant difference in the Mcl-1, caspase-3, caspase-8, caspase-9, p53, p21, or PCNA protein LS between the CR and PR/NR groups. <strong></strong></p><p> <strong>Conclusion</strong></p><p>  We conclude that the Bak LS or Bak/Mcl-1 LS ratio may be a useful biomarker to predict the clinical outcomes of OVH and OL lesions treated with topical ALA-PDT. Pre-PDT epithelial cell levels of Mcl-1, caspase-3, caspase-8, caspase-9, p53, p21, and PCNA may not have a significant influence on the clinical outcome of OVH and OL lesions treated with topical ALA-PDT. </p><hr><p></p><p> <strong>Source: </strong>Journal of Oral Pathology & Medicine</p><p> <a href="http://onlinelibrary.wiley.com/doi/10.1111/jop.12007/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> 5-aminolevulinic acid;clinical outcome;immunohistochemistry;oral leukoplakia;oral verrucous hyperplasia;photodynamic therapy 0 0 http://idai.ir/browse.php?a_code=A-10-32-2245&slc_lang=fa&sid=1 Chuan-Hang Yu 100319475328460010601 100319475328460010601 No School of Dentistry, College of Oral Medicine, Chung Shan Medical University, Taichung, Taiwan/Department of Dentistry, Oral Medicine Center, Chung Shan Medical University Hospital, Taichung, Taiwan Hsin-Ming Chen 100319475328460010602 100319475328460010602 No Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan/Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan/Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan Hung-Pin Lin 100319475328460010603 100319475328460010603 No Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan/Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan Chun-Pin Chiang cpchiang@ntu.edu.tw 100319475328460010604 100319475328460010604 Yes Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan/Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan/Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan