fa Human gingival fibroblast functions are stimulated by oxidized nano-structured titanium surfaces جراحی دهان، فک و صورت Oral and Maxillofacial Surgery پژوهشي Research <p> <strong>Objectives</strong></p><p>  The aim of this study was to analyze the features of an oxidized titanium implant surface and to evaluate its effects on the response of human gingival fibroblasts. </p><p> <strong>Methods</strong></p><p>  10mm×10mm×1mm turned (control) and oxidized (test) titanium samples (P.H.I. s.r.l., Italy) were examined by scanning electron microscopy and atomic force microscopy and characterized by height, spatial and hybrid roughness parameters. Primary cultures of human gingival fibroblasts were seeded on titanium samples, and cell morphology, adhesion, proliferation and extracellular matrix deposition, in terms of type I collagen synthesis, were evaluated. </p><p> <strong>Results</strong></p><p>  Control and test surfaces appeared considerably different at the microscopic analyses: turned samples were grooved, whereas oxidized surfaces showed a more complex micro- and nano-scaled texture, as evidenced by roughness parameters. Cell adhesion and proliferation rate, as well as collagen synthesis, were greater on oxidized vs turned surfaces. </p><p> <strong>Conclusions</strong></p><p>  Although both control and test samples were in the range of average roughness proper of smooth surfaces, they exhibited significantly different topographic properties in terms of height and, mostly, hybrid parameters. Furthermore, oxidized surfaces enhanced human gingival fibroblast adhesion, proliferation and extracellular matrix deposition, and this could be due to the different structure at micro- and nano-scale levels. </p><p> <strong>Clinical significance</strong></p><p>  Oxidized nanostructured titanium surfaces could have a significant clinical utilization in virtue of their affinity for soft tissue attachment at the implant neck and/or at the transmucosal portion of the prosthetic abutment. </p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry</p><p> <a href="http://www.jodjournal.com/article/S0300-5712(13)00182-6/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> Dental implant, Human gingival fibroblasts, Surface topography, Cell adhesion, Cell proliferation, Collagen synthesis 0 0 http://idai.ir/browse.php?a_code=A-10-32-2205&slc_lang=fa&sid=1 Luigi Guida 100319475328460010386 100319475328460010386 No Multidisciplinary Department of Medical–Surgical and Dental Specialties, Second University of Naples, Via L. De Crecchio, 6, 80138 Naples, Italy Adriana Oliva 100319475328460010387 100319475328460010387 No Department of Biochemistry and Biophysics “F. Cedrangolo”, Second University of Naples, Via L. De Crecchio, 7, 80138 Naples, Italy Maria Assunta Basile 100319475328460010388 100319475328460010388 No Department of Biochemistry and Biophysics “F. Cedrangolo”, Second University of Naples, Via L. De Crecchio, 7, 80138 Naples, Italy Michele Giordano 100319475328460010389 100319475328460010389 No Institute for Composite and Biomedical Materials, National Research Council (IMCB-CNR), Piazzale Enrico Fermi 1, 80055 Portici, Italy Livie Nastri 100319475328460010390 100319475328460010390 No Multidisciplinary Department of Medical–Surgical and Dental Specialties, Second University of Naples, Via L. De Crecchio, 6, 80138 Naples, Italy Marco Annunziata 100319475328460010391 100319475328460010391 Yes Multidisciplinary Department of Medical–Surgical and Dental Specialties, Second University of Naples, Via L. De Crecchio, 6, 80138 Naples, Italy