fa The inhibitory effect of proanthocyanidin on soluble and collagen-bound proteases کودکان Pediatric Dentistry پژوهشي Research <p> <strong>Objective</strong></p><p>  This study evaluated the inhibitory effect of proanthocyanidin (PA), a natural collagen cross-linker, on soluble and matrix-bound proteases, which are responsible for progressive degradation of exposed collagen fibrils within the hybrid layer and resin–dentine bond failure over time. <strong></strong></p><p> <strong>Methods</strong></p><p>  The inhibitory effects of PA (1%, 2%, 3%, 4.5% and 6%) on soluble recombinant matrix metalloproteinases (MMP-2, -8 and -9) and cysteine cathepsins (cathepsin B and K) were evaluated using MMP and cysteine cathepsins fluorometric assay kits. Chlorhexidine (CHX) was used as an inhibitor control. The effect of PA on endogenous matrix-bound proteases was examined by determining the change in dry mass of demineralized dentine beams and solubilized collagen peptides over 30 days. Two-way ANOVA and Tukey multiple comparison tests were used to analyze the effect of PA and proteases on the percentage inhibition of soluble proteases (α=0.05). Kruskal–Wallis one-way ANOVA and Dunn's multiple comparison tests were used to analyse the effect of PA on loss of dry mass and hydroxyproline content over time (α=0.05). </p><p> <strong>Results</strong></p><p>  Proanthocyanidin inactivated more than 90% of soluble recombinant MMP-2, -8 and -9 and around 75–90% of cysteine cathepsin B and K, which was significantly higher than CHX (P<0.05). The inhibition of endogenous proteases by PA increased in a dose-dependent manner. The loss of dry mass and hydroxyproline release in the medium over time was the lowest in dentine beams pretreated with PA<chx<control /> < CHX <(P<0.05).</p><p> <strong>Conclusion</strong></p><p> Proanthocyanidin exibited both dentine MMP cysteine cathepsins inhibition, which was higher than chlorhexidine.</p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry</p><p><a href="http://www.iphotoshop.ir/" target="_blank"><font color="#0000ff"> Full Text</font></a></p> Collagen cross-linking, Proanthocyanidin, Dentine, Matrix metalloproteinases, Cystein cathepsins 0 0 http://idai.ir/browse.php?a_code=A-10-32-2198&slc_lang=fa&sid=1 Don Jeevanie Epasinghe 100319475328460010336 100319475328460010336 No Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong, China Cynthia Kar Yung Yiu 100319475328460010337 100319475328460010337 Yes Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong, China Michael Francis Burrow 100319475328460010338 100319475328460010338 No Oral Diagnosis and Polyclinic, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong, China Noriko Hiraishi 100319475328460010339 100319475328460010339 No Cariology and Operative Dentistry, Department of Restorative Science, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, Japan Franklin Russell Tay 100319475328460010340 100319475328460010340 No Department of Endodontics, College of Dental Medicine, Georgia Health Sciences University, Augusta, GA, USA