fa Streptococcus mutans biofilm disruption by κ-casein glycopeptide پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Abstract</strong></p><p>  Caseinomacropeptide (CMP), the variably phosphorylated and glycosylated forms of the bovine milk protein fragment, κ-casein(106–169), is produced during cheese production and has been shown to have a range of antibacterial bioactivities. </p><p> <strong>Objectives</strong></p><p>  To characterise the biofilm disruptive component of CMP and compare its activity with the known antimicrobial agents chlorhexidine and zinc ions. </p><p> <strong>Methods</strong></p><p>  Streptococcus mutans biofilms were grown in flow cells with an artificial saliva medium containing sucrose and treated with CMP and the glycosylated forms of κ-casein(106–169) (κ-casein glycopeptide, KCG). The biofilms were imaged using confocal laser scanning microscopy (CLSM) and quantified by COMSTAT software analysis. A static biofilm assay and flow cytometric analysis were used to examine the mechanism of action of chlorhexidine and a combination of KCG with the known antimicrobial agent ZnCl2 (KCG-Zn).</p><p> <strong> Results</strong></p><p>  CLSM analysis showed that S. mutans produced robust, structured biofilms with an average thickness of 7.37μm and a biovolume of 3.88μm3/μm2 substratum after 16h of incubation in the flow cell system. A single application of 10mg/mL CMP that contained 2.4mg/mL KCG significantly reduced total biofilm biovolume and average biofilm thickness by 53% and 61%, respectively. This was statistically the same as a 2.4mg/mL KCG treatment that reduced the total biovolume and average thickness by 59% and 69%, respectively, suggesting the KCG was the biofilm disruptive component of CMP. Chlorhexidine treatment (0.1%) caused similar effects in the flow cell model. KCG-Zn caused significantly more disruption of the biofilms than either KCG or ZnCl2 treatment alone. In a static biofilm model chlorhexidine was shown to work by disrupting bacterial membrane integrity whilst KCG-Zn had no effect on membrane integrity. </p><p> <strong>Conclusions</strong></p><p>  KCG and KCG-Zn may have potential as natural biofilm disruptive agents. </p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry</p><p> <a href="http://www.jodjournal.com/article/S0300-5712(13)00090-0/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> Streptococcus mutans, Biofilm, Casein glycopeptide, Chlorhexidine 0 0 http://idai.ir/browse.php?a_code=A-10-32-2160&slc_lang=fa&sid=1 Stuart G. Dashper 100319475328460010144 100319475328460010144 No Sze-Wei Liu 100319475328460010145 100319475328460010145 No Katrina A. Walsh 100319475328460010146 100319475328460010146 No Geoffrey G. Adams 100319475328460010147 100319475328460010147 No David P. Stanton 100319475328460010148 100319475328460010148 No Brent R. Ward 100319475328460010149 100319475328460010149 No Peiyan Shen 100319475328460010150 100319475328460010150 No Neil M. O’Brien-Simpson 100319475328460010151 100319475328460010151 No Eric C. Reynolds 100319475328460010152 100319475328460010152 Yes Melbourne Dental School, The University of Melbourne, 720 Swanston Street, Melbourne, Victoria 3010, Australia