fa Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentine bond properties ارتودنسی Orthodontics پژوهشي Research <p> <strong>Objectives </strong></p><p> The objective of this study was to investigate the effects of dentine primer containing dual antibacterial agents, namely, 12-methacryloyloxydodecylpyridinium bromide (MDPB) and nanoparticles of silver (NAg), on dentine bond strength, dental plaque microcosm biofilm response, and fibroblast cytotoxicity for the first time. </p><p> <strong>Methods</strong></p><p>  Scotchbond Multi-Purpose (SBMP) was used as the parent bonding agent. Four primers were tested: SBMP primer control (referred to as “P”), P+5% MDPB, P+0.05% NAg, and P+5% MDPB+0.05% NAg. Dentine shear bond strengths were measured using extracted human teeth. Biofilms from the mixed saliva of 10 donors were cultured to investigate metabolic activity, colony-forming units (CFU), and lactic acid production. Human fibroblast cytotoxicity of the four primers was tested in vitro. </p><p> <strong>Results</strong></p><p>  Incorporating MDPB and NAg into primer did not reduce dentine bond strength compared to control (p>0.1). SEM revealed well-bonded adhesive–dentine interfaces with numerous resin tags. MDPB or NAg each greatly reduced biofilm viability and acid production, compared to control. Dual agents MDPB+NAg had a much stronger effect than either agent alone (p<0.05), increasing inhibition zone size and reducing metabolic activity, CFU and lactic acid by an order of magnitude, compared to control. There was no difference in cytotoxicity between commercial control and antibacterial primers (p>0.1). </p><p> <strong>Conclusions</strong></p><p>  The method of using dual agents MDPB+NAg in the primer yielded potent antibacterial properties. Hence, this method may be promising to combat residual bacteria in tooth cavity and invading bacteria at the margins. The dual agents MDPB+NAg may have wide applicability to other adhesives, composites, sealants and cements to inhibit biofilms and caries. </p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry</p><p><a href="http://www.jodjournal.com/article/S0300-5712(13)00039-0/abstract" target="_blank"><font color="#0000ff"> Full Text</font></a></p> Antibacterial primer, Dentine bond strength, Silver nanoparticles, MDPB, Human saliva microcosm biofilm, Caries inhibition 0 0 http://idai.ir/browse.php?a_code=A-10-32-2154&slc_lang=fa&sid=1 Ke Zhang 100319475328460010116 100319475328460010116 No Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA Lei Cheng 100319475328460010117 100319475328460010117 No Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA Satoshi Imazato 100319475328460010118 100319475328460010118 No Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan Joseph O.Antonucci 100319475328460010119 100319475328460010119 No Biomaterials Group, Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA aNancy J.Lin 100319475328460010120 100319475328460010120 No Biomaterials Group, Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA Sheng Lin-Gibson 100319475328460010121 100319475328460010121 No Biomaterials Group, Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA Yuxing Bai 100319475328460010122 100319475328460010122 Yes Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China Hockin H.K. Xu 100319475328460010123 100319475328460010123 No Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA