fa In vitro and in vivo investigation of the biological and mechanical behaviour of resin-modified glass-ionomer cement containing chlorhexidine کودکان Pediatric Dentistry پژوهشي Research <p> <strong>Objectives</strong> </p><p> To evaluate<strong>:</strong> (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT).</p><p> <strong> Methods</strong></p><p> <strong> </strong>For the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5% CHX. Microbiologic evaluation consisted of an agar diffusion test on cariogenic bacteria for 24h. Odontoblast-like cell metabolism and morphology analyses measured the cytotoxic effects of the RMGIC groups after 24h. The same groups were submitted to compressive and diametral tensile strength. The in vivo treatment consisted of IPT using an RMGIC associated with the best CHX concentration. Clinical and microbiologic evaluations were performed before and after 3 months. </p><p> <strong>Results</strong></p><p>  The use of 1.25% CHX significantly improved the antibacterial effects of the evaluated RMGIC, without causing any detrimental effects to the odontoblast-like cells and on the mechanical properties. This RMGIC and CHX combination completely eliminated mutans streptococci after 3 months of IPT. </p><p> <strong>Conclusion</strong></p><p> <strong> </strong>The RMGIC and 1.25% CHX mixture showed great biological and mechanical behaviour and could be a good treatment against caries progression. Clinical significance The association of CHX with a liner RMGIC opens a new perspective for arresting residual caries after IPT. </p><hr><p></p><p> <strong>Source: </strong>Journal of Dentistry</p><p><a href="http://www.jodjournal.com/article/S0300-5712(12)00298-9/abstract" target="_blank"> <font color="#0000ff">Full Text</font></a></p> Glass ionomer cement, Chlorhexidine digluconate, Cell culture, Antibacterial activity, Mechanical properties, Indirect pulp therapy 0 0 http://idai.ir/browse.php?a_code=A-10-32-2109&slc_lang=fa&sid=1 Aline Rogéria Freire de Castilho 10031947532846009884 10031947532846009884 No Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil Cristiane Duque 10031947532846009885 10031947532846009885 No Department of Pediatric Dentistry, Araçatuba School of Dentistry, University of São Paulo State (UNESP), Araçatuba, São Paulo, Brazil Thaís de Cássia Negrini 10031947532846009886 10031947532846009886 No Department of Clinical Analysis, Araraquara School of Pharmacy, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil Nancy Tomoko Sacono 10031947532846009887 10031947532846009887 No Department of Physiology and Pathology, Araraquara School of Dentistry, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil Andréia Bolzan de Paula 10031947532846009888 10031947532846009888 No Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil Carlos Alberto de Souza Costa 10031947532846009889 10031947532846009889 No Department of Physiology and Pathology, Araraquara School of Dentistry, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil Denise Madalena Palomari Spolidório 10031947532846009890 10031947532846009890 No Department of Physiology and Pathology, Araraquara School of Dentistry, University of São Paulo State (UNESP), Araraquara, São Paulo, Brazil Regina Maria Puppin-Rontani 10031947532846009891 10031947532846009891 Yes Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil