fa Stable gene-silence of Kif2a synergistic with 5-fluorouracil suppresses oral tongue squamous cell carcinoma growth in vitro and in vivo بیماری های دهان Oral Medicine پژوهشي Research <p> <strong>Objective</strong></p><p>  Squamous cell carcinoma of the oral tongue (SCCOT) is one of the most common malignant carcinomas in the head and neck. Recurrence and/or metastasis often results in failure of treatment and decreases the survival of the patients. The purpose of this study is to investigate the effect of gene-silence of Kif2a on SCCOT in viro and in vivo. </p><p> <strong>Study design</strong></p><p>  Plasmid-mediated expression of Kif2a-siRNA (pGPU6/GFP/Kif2a) was employed to silence the expression of Kif2a in Tca8113 cells at both mRNA and protein levels. Tca8113 cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and growth of Tca8113 tumors was determined by intra-tumor injection of pGPU6/GFP/Kif2a in nude mice. </p><p> <strong>Results</strong></p><p>  Gene-silence of Kif2a suppressed Tca8113 cell proliferation. pGPU6/GFP/Kif2a synergized the tumor suppression effect of 5-Fluorouracil (5-Fu) on Tca8113 cells. </p><p> <strong>Conclusions</strong></p><p>  Our data support that Kif2a is a potential molecular target for the therapeutics of recurrent and metastatic SCCOT. </p><hr><p></p><p> <strong>Source: </strong>Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology </p><p> <a href="http://www.oooojournal.net/article/S2212-4403(13)00059-X/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> 0 0 http://idai.ir/browse.php?a_code=A-10-32-1656&slc_lang=fa&sid=1 Cheng-Qin Wang 10031947532846007761 10031947532846007761 No Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China Yu-Jun Li 10031947532846007762 10031947532846007762 Yes Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China Zhi-Min Wei 10031947532846007763 10031947532846007763 No Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China Chang-Jun Zhu 10031947532846007764 10031947532846007764 No College of Life Science/Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin, China Xun Qu 10031947532846007765 10031947532846007765 No Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, Shandong, China Feng-Cai Wei 10031947532846007766 10031947532846007766 No Department of Stomatology, Qilu Hospital, Shandong University, Jinan, Shandong, China Xiao-Ming Xing 10031947532846007767 10031947532846007767 No Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China Wen-Juan Yu 10031947532846007768 10031947532846007768 No Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China