fa Assessing the contribution of HRPT2 to the pathogenesis of jaw fibrous dysplasia, ossifying fibroma, and osteosarcoma پاتولوزی دهان، فک و صورت Oral and Maxillofacial Pathology پژوهشي Research <p> <strong>Objective </strong></p><p> To investigate HRPT2 in jaw ossifying fibroma (OF), fibrous dysplasia (FD), and osteosarcoma (OS). </p><p> <strong>Study Design</strong> </p><p> We combined microsatellite loss of heterozygosity (LOH), HRPT2 sequence alterations at the mRNA level by reverse-transcription polymerase chain reaction (PCR), cDNA sequencing, and quantitative PCR (qPCR) and immunohistochemistry (IHC) in a total of 19 OF, 15 FD, and 9 OS. Because HRPT2 (parafibromin) interacts with cyclin D1, we investigated cyclin D1 expression with the use of qPCR and IHC. </p><p> <strong>Results</strong></p><p>  LOH was detected in 3/5 FD, 6/9 OF, and 2/2 OS heterozygous samples. LOH was not associated with decreased mRNA levels or HRPT2 protein expression except for 1 OF which harbored an inactivating mutation. However, this tumor did not display altered transcription or protein levels of HRPT2 nor cyclin compared with the other OF.</p><p> <strong> Conclusions</strong></p><p>  The contribution of HRPT2 inactivation to the pathogenesis of OF, FD, and OS is marginal at best and may be limited to progression rather than tumor initiation. </p><hr><p></p><p> <strong>Source: </strong>Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology </p><p> <a href="http://www.oooojournal.net/article/S2212-4403(12)01726-9/abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> 0 0 http://idai.ir/browse.php?a_code=A-10-32-1558&slc_lang=fa&sid=1 Ana Carolina de Masquita Netto 10031947532846007199 10031947532846007199 No Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Ricardo Santiago Gomez 10031947532846007200 10031947532846007200 No Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Marina Gonçalves Diniz 10031947532846007201 10031947532846007201 No Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Tiago Fonseca-Silva 10031947532846007202 10031947532846007202 No Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Kelma Campos 10031947532846007203 10031947532846007203 No Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Luiz De Marco 10031947532846007204 10031947532846007204 No Department of Surgery, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil Román Carlos 10031947532846007205 10031947532846007205 No Centro Clínico de Cabeza y Cuello, Hospital Herrera-Llerandi, Guatemala Carolina Cavaliéri Gomes 10031947532846007206 10031947532846007206 Yes Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil