fa A comparison of the thresholding strategies of micro-CT for periodontal bone loss: a pilot study رادیولوژی دهان، فک و صورت Oral and Maxillofacial Radiology پژوهشي Research <p><strong>Objectives:</strong> Micro-CT provides three-dimensional details and has been widely used for biomedical assessments. This study aimed to determine the most appropriate threshold method for quantitatively assessing the dynamics of periodontal destruction. </p><p><strong>Methods:</strong> Inflammation was induced by submerging a silk ligature in the sulcus of the maxillary second molars of rats, and the animals were killed prior to ligature placement and after 7 and 21 days. The maxillae were examined for the bone resorptive activities by micro-CT, histology and tartrate-resistant acid phosphatase staining. The imaging threshold was determined by CT phantom, global and local algorithms. A bone fraction measurement from each threshold-determining technique was compared with histomorphometry. The reliability and reproducibility were examined by the intraclass correlation coefficient (ICC) and the coefficient of variation. </p><p><strong>Results:</strong> Significant reduction of inflammatory infiltration (p < 0.01) and active osteoclastic resorption (p < 0.05) from Day 7 to Day 21 were noted. High inter- and intraexaminer agreement were demonstrated in both histomorphometric and micro-CT assessments (ICC > 0.98). The algorithm-based technique demonstrated stronger correlation to histomorphometry than phantom-based thresholds, and the highest agreement was presented by the local algorithm (ICC > 0.96). This, however, was considerably computationally expensive. </p><p><strong>Conclusions</strong>: The local threshold-determining algorithm is suggested for examining inflammation-induced bone loss. Further investigation will be aimed at enhancing computational efficiency. </p><hr><p></p><p><strong>Source: </strong>DentoMaxilloFacial Radiology </p><p><a href="http://dmfr.birjournals.org/content/42/2/66925194.abstract" target="_blank"><font color="#0000ff">Full Text</font></a></p> X-ray microtomography ,periodontitis ,histology 0 0 http://idai.ir/browse.php?a_code=A-10-32-1356&slc_lang=fa&sid=1 P-C Chang dencpc@nus.edu.sg 10031947532846006323 10031947532846006323 Yes Faculty of Dentistry, National University of Singapore, Singapore, Singapore K Liang 10031947532846006324 10031947532846006324 No Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology, and Research (A*STAR), Singapore, Singapore JC Lim 10031947532846006325 10031947532846006325 No Singapore Bioimaging Consortium (SBIC), Agency for Science, Technology, and Research (A*STAR), Singapore, Singapore M-C Chung 10031947532846006326 10031947532846006326 No Faculty of Dentistry, National University of Singapore, Singapore, Singapore L-Y Chien 10031947532846006327 10031947532846006327 No Faculty of Dentistry, National University of Singapore, Singapore, Singapore