en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background:</strong> The aim of this study is to evaluate the effects of caffeine and/or estrogen deficiency on ligature-induced bone loss (BL), trabecular bone area (TBA), and postextraction bone healing (BH). </p><p> <strong>Methods:</strong> Rats were assigned into one of the following groups (15 each): 1) control = non-ingestion of caffeine/sham surgery 2) caffeine = ingestion of caffeine/sham surgery) 3) ovariectomized (OVX) = non-ingestion of caffeine/ovariectomy or 4) caffeine/OVX = ingestion of caffeine/ovariectomy. The rats were under caffeine administration for 65 days and/or estrogen deficiency for 51 days. On day 21 after ovariectomy, one first mandibular molar received a ligature and the contralateral tooth was not ligated. The first maxillary molars were extracted 8 days before sacrifice. BL, TBA, the positive cells for tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG) were analyzed in the furcation area of mandibular molars. Histometric BH and gene expression of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin, and bone sialoprotein were evaluated in alveolar sockets. </p><p> <strong>Results:</strong> The caffeine group presented the greatest BL and the OVX group the highest number of TRAP-positive (TRAP+) cells around ligated teeth (P <0.05). The control group presented higher TBA and BH than the other groups (P <0.05). All test groups presented higher RANKL/OPG+ cells than the control group around ligated/unligated teeth. The OVX and caffeine/OVX groups presented a greater number of TRAP+ cells around unligated teeth than the control group (P <0.05). There were no differences among groups for gene expression (P >0.05). <strong></strong></p><p> <strong>Conclusions:</strong> Caffeine increased BL in ligated teeth. Caffeine and/or estrogen deficiency decreased TBA in the unligated teeth and reduced BH after tooth extraction. </p><hr><p></p><p> <strong>Source: </strong>Journal of Periodentology </p><p> <a href="http://www.joponline.org/doi/abs/10.1902/jop.2012.120192" target="_blank"><font color="#0000ff">Full Text</font></a></p> Alveolar bone loss, caffeine, osteoprotegerin, ovariectomy, RANK ligand 0 0 http://idai.ir/browse.php?a_code=A-10-32-1317&slc_lang=en&sid=1 Joyce Pinho Bezerra 10031947532846005854 10031947532846005854 No Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil Ariane de Siqueira 10031947532846005855 10031947532846005855 No Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil Amanda Gonçalves Pires 10031947532846005856 10031947532846005856 No Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil Marcelo Rocha Marques 10031947532846005857 10031947532846005857 No Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo, Brazil Poliana Mendes Duarte pduarte@ung.br 10031947532846005858 10031947532846005858 Yes Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil Marta Ferreira Bastos 10031947532846005859 10031947532846005859 No Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil