en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background:</strong> In chronic periodontitis (CP), the gene polymorphism of interleukin-6 (IL-6) to 174C/G has been associated with the altered production of this cytokine. The aim of this pilot study is to compare the allelic and genotypic frequencies in patients with CP with control individuals without periodontitis (NP) and to measure the production of IL-6 by whole blood cells stimulated with Porphyromonas gingivalis HmuY protein. </p><p> <strong>Methods:</strong> DNA was isolated from peripheral blood cells of 49 patients with CP and 60 control individuals classified as NP, and genotyping was performed by polymerase chain reaction using sequence-specific primers. Whole blood cells from 29 patients with CP and 30 control individuals were stimulated for 48 hours with HmuY, and IL-6 levels were measured using enzyme-linked immunosorbent assay. </p><p> <strong>Results:</strong> The proportion of individuals carrying the G allele at position –174 of the IL-6 gene was higher in the group with CP (85.7%) than in the normal control group (73.3% P <0.03). P. gingivalis HmuY-induced production of IL-6 was higher in the group with CP (P <0.05). <strong></strong></p><p> <strong>Conclusions</strong>: Our findings suggest that P. gingivalis HmuY may be associated with increased IL-6 production during CP. Furthermore, patients with periodontitis and individuals with higher HmuY-induced production of IL-6 show a high frequency of the G allele at position –174. </p><hr><p></p><p> <strong>Source: </strong>Journal of Periodentology </p><p> <a href="http://www.joponline.org/doi/abs/10.1902/jop.2012.120230" target="_blank"><font color="#0000ff">Full Text</font></a></p> Interleukin-6, periodontitis, Porphyromonas gingivalis 0 0 http://idai.ir/browse.php?a_code=A-10-32-1296&slc_lang=en&sid=1 Soraya Castro Trindade soraya@uefs.br 10031947532846005735 10031947532846005735 Yes Department of Immunology, Federal University of Bahia, Bahia, Brazil/Department of Periodontics, Feira de Santana State University, Bahia, Brazil Teresa Olczak 10031947532846005736 10031947532846005736 No Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland Issac Suzart Gomes-Filho 10031947532846005737 10031947532846005737 No Department of Periodontics, Feira de Santana State University, Bahia, Brazil Lília Ferreira de Moura-Costa 10031947532846005738 10031947532846005738 No Department of Immunology, Federal University of Bahia, Bahia, Brazil Vera Costa Vale 10031947532846005739 10031947532846005739 No Department of Microbiology, Bahia State University, Bahia, Brazil Milton Galdino-Neto 10031947532846005740 10031947532846005740 No Department of Immunology, Federal University of Bahia, Bahia, Brazil Heidiane Alves dos Santos 10031947532846005741 10031947532846005741 No Department of Immunology, Federal University of Bahia, Bahia, Brazil Paulo Cirino de Carvalho Filho 10031947532846005742 10031947532846005742 No Department of Immunology, Federal University of Bahia, Bahia, Brazil Andreas Stocker 10031947532846005743 10031947532846005743 No Department of Immunology, Federal University of Bahia, Bahia, Brazil Maria Teresita Bendicho 10031947532846005744 10031947532846005744 No Department of Immunology, Federal University of Bahia, Bahia, Brazil