en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background</strong>: It has been suggested that cyclosporine A (CsA) induces gingival enlargement by promoting an increase in the gingival extracellular matrix (ECM). Nonetheless, the variable occurrence of CsA-induced gingival enlargement in patients receiving this medication indicates a multifactorial pathogenesis. Clinical observations suggest that local inflammation is associated with the development and severity of CsA-induced gingival enlargement. Therefore, the purpose of this study is to investigate the effects of CsA and inflammation on the production of ECM homeostatic mediators. </p><p> <strong>Methods:</strong> The effects of CsA and inflammation (as assessed using interleukin [IL]-1β) on the secretion of mediators involved in ECM homeostasis were determined using fibroblast monolayers and three-dimensional (3D) models of the human oral mucosa. Fibroblast monolayers and 3D cultures were treated with CsA alone or in combination with IL-1β for up to 72 hours, and the secretion of matrix metalloproteinases (MMPs) 1, 2, 3, 8, 9, 10, and 13 and tissue inhibitors of MMPs (TIMPs) 1, 2, and 4 into the culture medium was assessed using enzyme-linked immunoassay–based antibody arrays. </p><p> <strong>Results:</strong> Fibroblast monolayers responded to CsA with no changes in the secretion of ECM mediators. Conversely, 3D cultures responded to CsA treatment with a reduction in MMP-10 secretion. IL-1β alone triggered higher secretory levels of MMPs in both fibroblast monolayers (MMP-3 and MMP-10) and 3D cultures (MMP-9 and MMP-10). Importantly, fibroblast monolayers and 3D cultures treated with a combination of IL-1β and CsA showed a decrease in the MMP-1/TIMP-1 ratio. </p><p> <strong>Conclusions:</strong> These data support the hypothesis that inflammation may alter the pathogenesis of CsA-induced gingival enlargement by promoting a synergistic decrease in the MMP-1/TIMP-1 ratio. </p><hr><p></p><p> <strong>Source: </strong>Journal of Periodentology </p><p><a href="http://www.joponline.org/doi/abs/10.1902/jop.2012.120224" target="_blank"><font color="#0000ff"> Full Text</font></a></p> Collagen, cyclosporine, gingival overgrowth, inflammation, metalloproteases, tissue inhibitor of metalloproteinases 0 0 http://idai.ir/browse.php?a_code=A-10-32-1294&slc_lang=en&sid=1 Matthew Johanson 10031947532846005724 10031947532846005724 No Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas Xiang R. Zhao 10031947532846005725 10031947532846005725 No Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas Guy Huynh-Ba 10031947532846005726 10031947532846005726 No Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas Cristina C.Villar villar@uthscsa.edu 10031947532846005727 10031947532846005727 Yes Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas