en پریودنتولوژی Periodontology پژوهشي Research <p> <strong>Background:</strong> The antimicrobial peptide LL-37 is known to have a potent lipopolysaccharide (LPS)-neutralizing activity in various cell types. Because of observed heterogeneity within periodontopathogenic LPS, the authors hypothesized that LL-37 had specificity to neutralize such LPS activity. The present study, therefore, aims to investigate the LPS-neutralizing activity of LL-37 to various periodontopathogenic LPS in interleukin-8 (IL-8) production after challenging them in human oral fibroblasts. </p><p> <strong>Methods:</strong> Human periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) were cultured from biopsies of periodontal ligament and gingival tissues. After cell confluence in 24-well plates, LPS (10 μg/mL) from Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans were added with or without LL-37 (10 μg/mL). After 18 hours, the supernatant was collected and analyzed in IL-8 production by enzyme-linked immunosorbent assay. </p><p> <strong>Results:</strong> All periodontopathogenic LPS statistically significantly induced IL-8 production in both PDLF and GF (P <0.01). After neutralization with LL-37, both PDLF and GF showed a statistically significant reduction in IL-8 production compared with LPS-treated groups without LL-37 (P <0.01), and the percentage of reduction in IL-8 production in PDLF appeared to be higher than in GF. In addition, the percentage of reduction in IL-8 production varied considerably according to each periodontopathogenic LPS. </p><p> <strong>Conclusions</strong>: The antimicrobial peptide LL-37 had an ability to suppress periodontopathogenic LPS-induced IL-8 production in both PDLF and GF. Its LPS-neutralizing activity revealed specificity to periodontopathogenic LPS and seemed to be dependent on the heterogeneity within LPS between different genera. </p><hr><p></p><p> <strong>Source: </strong>Journal of Periodentology </p><p><a href="http://www.joponline.org/doi/abs/10.1902/jop.2012.110652" target="_blank"><font color="#0000ff"> Full Text</font></a></p> Cathelicidin antimicrobial peptide, fibroblasts, lipopolysaccharides 0 0 http://idai.ir/browse.php?a_code=A-10-32-1258&slc_lang=en&sid=1 Wiroj Suphasiriroj bukung@ngt.ndu.ac.jp 10031947532846005505 10031947532846005505 Yes Department of Periodontology, The Nippon Dental University, School of Life Dentistry at Niigata, Niigata, Japan Masato Mikami 10031947532846005506 10031947532846005506 No Department of Microbiology, The Nippon Dental University, School of Life Dentistry at Niigata Hiromi Shimomura 10031947532846005507 10031947532846005507 No Department of Biochemistry, The Nippon Dental University, School of Life Dentistry at Niigata Soh Sato 10031947532846005508 10031947532846005508 No Department of Periodontology, The Nippon Dental University, School of Life Dentistry at Niigata, Niigata, Japan