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جشنواره سال 1393: نفرات برتر مقاله پژوهشی زیست مواد دندانی

نفرات برتر مقاله پژوهشی زیست مواد دندانی

 .

نفر اول مقاله پژوهشی زیست مواد دندانی

  Effect of dentine matrix proteins on human endometrial adult stem-like cells: In vitro regeneration of odontoblasts cells

 

  Fahimeh S. Tabatabaei , Jafar Ai , Tahereh S. Jafarzadeh Kashi * , Mozaffar Khazaei , Abdol-Mohammad Kajbafzadeh , Zinat Ghanbari

  * Tehran University of Medical Sciences, Faculty of Dentistry, Department of Dental Materials, Tehran, Iran


  

  Abstract

  Objective: Human endometrium has enormous regenerative capacity due to the presence of endometrial stem cells. The present study sought to assess the possibility of differentiation of these cells into odontoblast-like cells by in vitro induction.

  Methods: Endometrial stem-like cells were obtained using enzymatic digestion of the biopsy samples of the endometrium after hysterectomy and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) which contained dentine non-collagenous proteins (dNCPs). The results were evaluated using the following assays: analysis of morphology, enzymatic assay for measuring alkaline phosphatase activity, Alizarin Red staining to detect in vitro formation of mineralized nodules, immunofluorescence for detection of dentine sialoprotein (DSP) and dentine-matrix protein (DMP1), and Western blotting for DMP1 expression.

  Results: Following induction, endometrial stem-like cells demonstrated high alkaline phosphatase activity, and expression of DSP and DMP1 confirmed the odontoblast phenotype. DMP1 level increased in samples treated with dNCPs.

  Conclusions: Study results indicated that odontoblastic differentiation of endometrial stem cells can be induced by extracellular matrix proteins (e.g. dNCPs). The capacity of endometrial stem-like cells to differentiate into odontoblast-like cells under specific conditions gives new insights into the mechanism of odontogenesis and highlights the potential of such approaches for further research on dental tissue regeneration.

  Keywords: Dentine non-collagenous proteins , Differentiation , Endometrial stem cells , Odontoblast


  Archives of oral biology 58 (2013) 871-879

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نفر دوم مقاله پژوهشی زیست مواد دندانی

 Synthesis, Characterization, and In Vitro Bioactivity of Sol-Gel-Derived SiO2–CaO–P2O5–MgO-SrO Bioactive Glass

  Yashar Rezaei , Fathollah Moztarzadeh , Sima Shahabi , Mohammadreza Tahriri


Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 44:692–701, 2014


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