Evaluation of inhibitory effects of cuminum cyminum oil on the fluconazaole resistant and susceptible Candida albicans isolated from HIV patients in Iran
Salari S * , Khosravi A.R, Katiraee F , Ayatollahi Mousavi S.A , Shokri H , Nikbakht Borujeni GH
* Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran and Assistant Professor, Faculty of Veterinary Medicine, University of Mazandaran, Mazandaran, Iran.
Oropharyngeal Candidiasis (OPC) continues to be considered the most common opportunistic fungal disease in HIV/AIDS patients globally. The present study was undertaken to determine the antifungal susceptibility of Candida species isolates were obtained from Iranian PLWH (people living with HIV) and cultured on CHROMagar and Sabouraud’s dextrose agar. All isolates were identified according to assimilation profile, germ tube, colony color and other conventional methods. Disk diffusion testing and Broth Micro dilution of Fluconazole according to the methods described in CLSI was performed. In addition, Cuminum cyminum essential oil was used to evaluation in vitro activity its against against fluconazole resistant and susceptible Candida albicans .In our study, C. albicans (50.2%) and C. glabrata (22%) were the most frequent isolated, from these isolates, 25.7% were resistant to fluconazole (MIC 64 μg/ml. Complement data showed mean MIC, 0.575% ± 0.6810% (range: 0.25%-2%) for Cuminum cyminum essential oil to Fluconazole-resistant Candida albicans isolates and mean MIC, 0.306% ± 0.2640% (Range, 0.125%-0.5%) for susceptible Candida albicans isolates with significantly difference (p<0.001) .Based on our result we conclude that screening of resistance candida isolates in clinical laboratory is idealistic for surveillance of antifungal resistance to patient’s managements, And Cuminum cyminum essential oil having antifungal properties, can be helpful to treat of candidiasis.
Keywords: Oral candidiasis , HIV patient , Candida species , Azoles resistance isolates , Cuminum cyminum cyminum essential oil
Journal of American Science, 2012;8(5)