Effect of Chlorhexidine Pretreatment on the Stability of Salivary Lactobacilli Probiotic in Six- to Twelve-Year-Old Children : A Randomized Controlled Trial
Naser Asl- Aminabadi ,* Leila Erfanparast , Akram Ebrahimi , Sina Gertasi- Oskouei.
* Department of Pediatric Dentistry, Tabriz University of Medical Sciences, Iran
This study aimed to investigate the efficacy of chlorhexidine mouthwash disinfection on the stability of lactobacilli probiotic. This study involved 105 children in 3 groups. Subjects in group A received chlorhexidine for 2 weeks, subjects of group B received probiotic yogurt containing Lactobacillus rhamnosus GG (LGG) at a concentration of 2 × 10(8) colony-forming units per gram for 3 weeks, and subjects in group C received chlorhexidine in the same manner as group A and 24 h after cessation, they received probiotic yogurt in the same manner as group B. Salivary Streptococcus mutans (SM) and LGG were counted at baseline, 24 h, 1 week and 5 weeks after each intervention in all groups. The SM count was reduced 24 h after cessation of chlorhexidine in group A (p < 0.001), and was steady for 5 weeks (p < 0.05). In group B, SM counts did not show significant differences 5 weeks after ending the intake of the probiotic (p > 0.05); LGG counts increased (p < 0.001), but were not steady over 5 weeks. In group C, a significant reduction in SM count was recorded, and the reduced count was steady for 5 weeks; a significant increase in LGG counts was registered (p < 0.001), which was steady for 5 weeks. Intergroup comparison showed a significant increase in LGG counts in groups B and C; however, this increase was not steady in group B. Chlorhexidine treatment before probiotic intake induced more stable colonization with LGG strains than probiotic alone.
Caries Res. 2011;45(2):148-54.