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پریودنتولوژی: Bone augmentation potential in rabbit calvaria and ex vivo cytotoxicity of four bone substituting materials

Bone augmentation potential in rabbit calvaria and ex vivo cytotoxicity of four bone substituting materials

Authors: Farzin Ghanavati1* • Gholam Ali Gholami2 • Saeid Asgari3 • Mohhammad Jafar Eghbal4 • Farzam Ghanavati5 • Hesam Rahimi 6 • Milad Karamlou7 • Mahmoud Tamizi8

1-Fellowship, Department of Periodontology & Implant, Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

2-Department of Periodontology, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran 

3-Department of Endodontics, Endodontic Research Center, Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

4-Department of Endodontics, Endodontic Research Center, Iranian Center for Dental Research, School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

6-Orthodontic Graduate Program, Harvard School of Dental Medicine, Boston, MA, USA

7-School of Dentistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

8-Department of Periodontology, Dental School, Mashhad University of Medical Sciences, Mashhad, Iran

 fghanavati@yahoo.com


 Abstract 

Background and aims.

 Tricalcium Phosphate (TCP), Bovine-Derived Hydroxyapatite (BioOss™), Demineralised Freeze-dried Bone Allograft (DFDBA) and Calcium Sulphate (CaS) were compared in vitro for osteoblast cytotoxicity and in rabbit’s calvaria to measure the bone histopathologic response.

Materials and methods.

 34 critical size defects in the calvaria of 12 male Australian rabbits were randomly filled with the materials and 2 empty defects were used as controls. After one month, histologicalal evaluation was performed on the samples to record regenerated bone type and volume, material absorption and the amount of inflammation. Saos-2 cell line was exposed to the materials and the cell line vitality was tested with Methyl Tetrazolium Test (MTT) to determine material’s osteoblast cell cytotoxicity.

Results.

 The type of regenerated bone did not show a significant difference between the groups (p=1.0) while the amount of bone inflammation was significantly different (p=0.021), where BioOss caused the least and DFDBA had the highest. Bone formation was also similar between the groups (p=0.428). DFDBA group showed the highest material absorption while TCP group had the lowest (p=0.028). DFDBA was associated with significantly higher Saos-2 cell line viability than TCP and BioOss that were significantly less cytotoxic comparing to Cas       (p< 0.0001).

Conclusion.

 DFDBA group had the highest amount of material absorption and was associated with more inflammation than other materials in the rabbit calvaria. BioOss exhibited lowest amount of inflammation and TCP had the lowest amount of material absorption. Results of cytotoxicity test might be affected by different solubility constants of the test materials.


ْSource: Journal of Periodontology & Implant Dentistry

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